Comparison of the Modulatory Effect of Ketotifen, Sodium Cromoglycate, Procaterol and Salbutamol in Human Skin, Lung and Tonsil Mast CellsOkayama Y. · Church M.K.
Immunopharmacology Group, Clinical Pharmacology, Southampton General Hospital, Southampton, UK
To assess the influence of mast cell heterogeneity on the inhibition of mediator release by drugs, the effects of ketotifen, sodium cromoglycate and β-adrenoceptor agonists were examined against IgE-dependent histamine and prostaglandin D2 release from enzymatically dispersed human skin, lung and tonsil-lar mast cells. At high concentrations, ketotifen was an inhibitor of histamine and prostaglandin D2 release from lung and tonsillar mast cells. No cross-tachyphylaxis with sodium cromoglycate was seen. In skin mast cells no inhibition of mediator release was observed with 1.0 μM ketotifen, above which histamine release was induced. Sodium cromoglycate was a weak inhibitor of histamine and prostaglandin D2 release from lung and tonsillar mast cells and showed tachyphylaxis. Sodium cromoglycate did not inhibit histamine and prostaglandin D2 release from skin mast cells. On the other hand, no heterogeneity was observed with the β-adrenoceptor agonists, procaterol and salbutamol. β-Adrenoceptor stimulants were significantly more effective in inhibiting prostaglandin D2 than histamine release. No tachyphylaxis was seen with prolongation of the incubation time before challenge. Our results suggest that human mast cells are heterogeneous with respect to the modulation of mediator release by ketotifen and sodium cromoglycate but not β-adrenoceptor agonists.
© 1992 S. Karger AG, Basel
Correspondence to: Dr. Yoshimichi Okayama, Immunopharmacology Group, Clinical Pharmacology, Centre Block, Southampton General Hospital, Southampton SQ9 4XY (UK)
Published online: September 02, 2009
Number of Print Pages : 10
International Archives of Allergy and Immunology
Vol. 97, No. 3, Year 1992 (Cover Date: 1992)
Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)
For additional information: http://www.karger.com/IAA