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Vol. 100, No. 1, 1993
Issue release date: 1993
Section title: Original Paper
Int Arch Allergy Immunol 1993;100:60–67
(DOI:10.1159/000236389)

Characterisation of Dog Allergens by Means of Immunoblotting

Spitzauer S.a · Schweiger Ch.a · Anrather J.c · Ebner Ch.b · Scheiner O.b · Kraft D.b · Rumpold H.a
aInstitute of Clinical Chemistry and Laboratory Diagnostics, bInstitute of General and Experimental Pathology, and cClinic of Veterinary Medicine, Department of Internal Medicine, University of Vienna, Austria

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 9/2/2009
Issue release date: 1993

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Sera from 75 patients with clinical type I allergy against dogs were investigated by means of immunoblotting using extracts prepared from dog hair/dander (CAN XI D) and saliva. In addition, selected sera were tested on extracts made of hair, skin, salivary glands (parotis and submandibularis), serum and liver. A 69-kD IgE-binding protein was identified in all extracts tested with an incidence of approximately 40% and shown to be dog albumin by means of inhibition experiments. In 96% of patients’ sera IgE antibodies reactive with a 19-kD and/or a 23-kD protein of the hair/dander extract (CAN XID) were observed. IgE binding to a 23-kD band was also detected in the hair and saliva extracts, but not in skin, salivary gland, serum and liver extracts. A 19-kD IgE-binding protein was strongly expressed in skin and to a lesser degree in saliva, but not in hair, serum and liver. Preincubation of patients sera with the hair extract and subsequent probing with the hair/dander extract (CAN XI D) inhibited IgE binding to the 23 kD protein whereas preincubation with the skin extract abolished IgE binding to the 19-kD protein. Using the hair/dander extract as inhibitor, IgE binding to the 19- and 23-kD proteins of saliva was abrogated. Thus it is concluded that the 23-kD protein is preferentially expressed in hair and saliva whereas the 19-kD protein is found in saliva and skin. Furthermore these two proteins are likely to represent immunologically independent major allergens.

© 1993 S. Karger AG, Basel


  

Author Contacts

Correspondence to: Dr. H. Rumpold, Klin. Inst. med. u. chem. Labordiagnostik, Währinger Gürtel 18–20, A–1090 Wien (Austria)

  

Article Information

Published online: September 02, 2009
Number of Print Pages : 8

  

Publication Details

International Archives of Allergy and Immunology

Vol. 100, No. 1, Year 1993 (Cover Date: 1993)

Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 9/2/2009
Issue release date: 1993

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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