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Vol. 103, No. 3, 1994
Issue release date: 1994
Section title: Short Communication
Int Arch Allergy Immunol 1994;103:307–310
(DOI:10.1159/000236645)

Inhibition of Enteropathogenic Escherichia coli (EPEC) Adhesion to HeLa Cells by Human Colostrum: Detection of Specific slgA Related to EPEC Outer-Membrane Proteins

Camara L.M. · Carbonare S.B. · Silva M.L.M. · Carneiro-Sampaio M.M.S.
aDepartment of Immunology, lnstituto de Ciências Biomédicas, Universidade de São Paulo, bDepartment of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil

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Article / Publication Details

First-Page Preview
Abstract of Short Communication

Published online: 9/3/2009

Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Human colostrum and a high molecular weight colostrum fraction (HMWF; > 14,000D) prevented the adhesion of localized adherent (LA+) O111:H––enteropathogenic Escherichia coli (EPEC) to HeLa cells. This effect was abolished after absorption with an O111:H––LA+ EPEC strain, but absorption with a LA- strain of same serotype had no effect on the process. A low molecular weight fraction (< 14,000 D), absorbed or not with LA+ or LA–– bacterial strains, did not inhibit the adherence of E. coli to HeLa cells. IgA-depleted colostrum had no inhibitory effect on bacterial adhesion, demonstrating the critical role of this protein in the phenomenon. Heat inactivation of whole colostrum did not significantly modify the inhibition adherence levels. Immunoblots of O111:H––LA+ strain outer-membrane complex reacted with colostrum and HMWF showing that IgA antibodies were predominantly reactive with a 94-kD protein. These data confirm and extend observations about colostrum slgA participation in adhesion inhibition of EPEC to HeLa cells and its response to a 94-kD outer-membrane protein.


  

Author Contacts

Correspondence to: Dr. Magda M.S. Carneiro-Sampaio, Departamento de Imunologia, lnstituto de Ciências Biomédicas, 05508-900 Universidade de São Paulo, São Paulo (Brazil)

  

Article Information

Published online: September 03, 2009
Number of Print Pages : 4

  

Publication Details

International Archives of Allergy and Immunology

Vol. 103, No. 3, Year 1994 (Cover Date: 1994)

Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Article / Publication Details

First-Page Preview
Abstract of Short Communication

Published online: 9/3/2009

Number of Print Pages: 4
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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