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Vol. 104, No. 4, 1994
Issue release date: 1994
Section title: Short Communication
Int Arch Allergy Immunol 1994;104:399–404
(DOI:10.1159/000236698)

Characterization of the 18-kDa Apple Allergen by Two-Dimensional Immunoblotting and Microsequencing

Vieths S.a · Schöning B.a · Petersen A.b
aInstitute of Food Chemistry, Technische Universität Berlin, and bForschungsinstitut Borstel, Division of Allergology, Borstel, Germany

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Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 11/24/1993
Accepted: 3/23/1994
Published online: 9/3/2009
Issue release date: 1994

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

A low-temperature extract taken from Golden Delicious apples was separated by two-dimensional polyacrylamide gel electrophoresis. By means of two-dimensional immunoblotting with patients’ serum containing IgE specific to Bet v I, a rabbit polyclonal antiserum raised against Bet v I, and two Bet v I specific monoclonal antibodies, epitopes cross-reactive to Bet v I were identified on an apple allergen with a molecular mass of 18 kDa and pI 5.5. Furthermore, certain antibody reactivities with 4 isoproteins of a molecular mass of 16 kDa and pIs ranging from 4.9 to 5.5 were observed, which may indicate the presence of Bet v I related epitopes on these proteins. Based on 26 amino acid residues, N-terminal sequencing of the 18-kDa apple allergen revealed 62% sequence identity between Bet v I from birch pollen and the apple allergen. Our results therefore support the view that both proteins express common as well as non-related IgE-reactive epitopes.

© 1994 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 11/24/1993
Accepted: 3/23/1994
Published online: 9/3/2009
Issue release date: 1994

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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