Expression of the Cutaneous Lymphocyte-Associated Antigen in Circulating T Cells in Drug-Allergic ReactionsGonzalez F.J. · Carvajal M.J. · Leiva L. · Juarez C. · Blanca M. · Santamaria L.F.
Research Unit for Allergic Diseases, Internal Medicine Department, Carlos Haya Hospital, Malaga, Spain
Background: Mechanisms underlying the production of delayed cutaneous reactions to drugs are poorly characterized. The cutaneous lymphocyte-associated antigen (CLA) is a skin-homing T cell receptor that defines T lymphocytes associated with the cutaneous immune response. We studied the percentage and activation phenotype of circulating CLA+ T cells in drug allergic patients and healthy controls. Methods: PBMCs were isolated from heparinized venous blood by Ficoll density gradient. Lymphocytes were stained for flow cytometry with anti-CLA, anti-CD3 and anti-HLA-DR mAbs. Five-parameter analysis was performed on an Ortho Cytoron Absolute flow cytometer. Results: We found increased percentages of circulating CLA+ T cells in drug- allergic patients compared to controls. Moreover, CLA+ T cells from drug-allergic individuals expressed a higher percentage of the T cell activation marker HLA-DR. Conclusions: These results suggest that CLA+ T cells may play a role in the pathology of delayed cutaneous reactions to drugs. Further studies are in progress to elucidate the role of skin-homing T cells in allergic reactions to drugs.
© 1997 S. Karger AG, Basel
Correspondence to: Dr. Carlos Juarez, Research Unit for Allergic Diseases, Internal Medicine Department, Carlos Haya Hospital, E–29010 Malaga (Spain)
Published online: September 04, 2009
Number of Print Pages : 3
International Archives of Allergy and Immunology
Vol. 113, No. 1-3, Year 1997 (Cover Date: 1997)
Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)
For additional information: http://www.karger.com/IAA