The influence of azithromycin on biofilm formation by Pseudomonas aeruginosa
, a cause of refractory chronic respiratory tract infection, was investigated. Alginic acid produced by a mucoid strain of P. aeruginosa
was quantified by high-performance liquid chromatography from colonies growing on an agar medium. Polysaccharides in the biofilm formed on silicon chips by a nonmucoid strain were determined by a tryptophan reaction. The effect of azithromycin was examined at concentrations below the minimum inhibitory concentration (sub-MIC) for each strain. Azithromycin significantly inhibited the production of alginic acid from the mucoid strain at ≥ 1/256 MIC, and the production of exopolysaccharides from the nonmucoid strain at ≥ 1/16 MIC. The inhibition of biofilm formation by azithromycin was also observed by scanning electron microscopy. These findings suggest that azithromycin inhibits biofilm formation by P. aeruginosa
at concentrations well below the MIC.
Masaru Nasu, MD, PhD, Second Department of Internal Medicine, Oita Medical University, Hasama-machi, Oita 879-55 (Japan)
Published online: September 11, 2009
Number of Print Pages : 6
Chemotherapy (International Journal of Experimental and Clinical Chemotherapy)
Vol. 42, No. 3, Year 1996 (Cover Date: 1996)
Journal Editor: Sörgel F. (Nürnberg-Heroldsberg)
ISSN: 0009-3157 (Print), eISSN: 1421-9794 (Online)
For additional information: http://www.karger.com/CHE
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