Expression of Th1 and Th2 Immunoregulatory Cytokines by Human EosinophilsWoerly G. · Roger N. · Loiseau S. · Capron M.
INSERM U167, Institut Pasteur, and University of Lille II, Lille, France
Besides cytotoxic mediators, human eosinophils can produce proinflammatory and anti–inflammatory cytokines, as well as growth factors and chemokines. The demonstration that eosinophils from patients could produce IL–5, IL–4 and IL–2 suggested their participation in the regulation of immune response. In the present work, we have examined the presence of Th1 (IFN–γ, IL–2) and Th2 (IL–4, IL–5, IL–10 and IL–13) cytokines in eosinophils from patients or donors by intracellular flow cytometry, and by immunocytochemistry. Whereas almost 100% eosinophils expressed intracellular IFN–γ, IL–2 and IL–10, the expression of IL–4, IL–5 and IL–13 is more variable and increased in patients versus donors. The differential release by eosinophils of Th2 versus Th1 cytokines is suggested both by the decrease of the intracellular content after culture, restricted to IL–4, IL–5 and IL–13, associated with an accumulation of IL–4 and IL–5 in the presence of Brefeldin. These results indicate that, through the preferential release of Th2 cytokines, human eosinophils can participate in the polarization of the immune response.
Correspondence to: Prof. Monique Capron
INSERM U167, Institut Pasteur de Lille, 1, rue du Professeur–Calmette
BP 245, F–59019 Lille Cedex (France)
Tel. +33 (0)3 20 87 79 62, Fax +33 (0)3 20 87 78 88
Number of Print Pages : 3
Number of Figures : 0, Number of Tables : 0, Number of References : 12
International Archives of Allergy and Immunology
Vol. 118, No. 2-4, Year 1999 (Cover Date: February-April 1999)
Journal Editor: D. Kraft, Vienna
ISSN: 1018–2438 (print), 1423–0097 (Online)
For additional information:http://www.karger.com/journals/iaa