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Table of Contents
Vol. 24, No. 5-6, 2009
Issue release date: November 2009
Section title: Original Paper
Cell Physiol Biochem 2009;24:547–556
(DOI:10.1159/000257511)

Gene Expression Patterns in Visceral and Subcutaneous Adipose Depots in Rats are Linked to Their Morphologic Features

Palou M. · Priego T. · Sánchez J. · Rodríguez A.M. · Palou A. · Picó C.
Molecular Biology, Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands (UIB), and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Palma de Mallorca
email Corresponding Author

Prof. Andreu Palou

Universidad de las Islas Baleares

Edificio Mateu Orfila

Cra. Valldemossa Km 7.5, Palma de Mallorca 07122 (Spain)

Tel. +34 971173170, Fax: +34 971173426, E-Mail andreu.palou@uib.es

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Abstract

The aim was to characterize the expression pattern of genes involved in lipid metabolism in internal (retroperitoneal, mesenteric) and subcutaneous (inguinal) adipose tissue depots in rats and their relation with site-specific morphological- and metabolic-features. Gene expression by RT-qPCR, western blot and morphometric analyses were performed. Lipogenesis-related genes (PPARγ2, SREBP1c, ACC1, GPAT, LPL, CD36, GLUT4) showed higher mRNA levels in the retroperitoneal depot versus the mesenteric and the inguinal depots; the expression of PPARgamma;2, ACC1, CD36, and GLUT4 in the mesenteric depot was also higher than in the inguinal depot. HKII was similarly expressed in the retroperitoneal and mesenteric depots and higher than in the inguinal one. The expression of lipolysis-related genes (HSL, ATGL) was higher in the retroperitoneal than in the mesenteric and inguinal depots, while the expression of fatty-acid oxidation-related genes (PPARα, CPT1) was lower in the retroperitoneal depot compared with the mesenteric and the inguinal depots. Thus, a higher expression of lipogenesis- and lipolysis-related genes and lower expression of fatty-acid oxidation-related genes in internal depots (particularly in the retroperitoneal, which also presents the largest adipocyte size) can explain its higher triacylglyceride turnover rates and hence account for the differential behavior of fat depots in physiological situations and its involvement in obesity-linked metabolic disorders.

© 2009 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: July 14, 2009
Published online: November 04, 2009
Issue release date: November 2009

Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: http://www.karger.com/CPB


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