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Vol. 37, No. 5, 2000
Issue release date: September–October 2000
Section title: Research Paper
J Vasc Res 2000;37:372–380
(DOI:10.1159/000025753)

Gene Transfer to Intact Mesenteric Arteries by Electroporation

Martin J.B. · Young J.L. · Benoit J.N. · Dean D.A.
Departments of aPhysiology and bMicrobiology and Immunology, University of South Alabama College of Medicine, Mobile, Ala., USA

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Published online: 10/2/2000

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A specially designed electroporation probe was placed around the neurovascular bundle and the electroporation chamber filled with a solution containing the firefly luciferase expressing plasmid (pCMV-Lux-DTS) or the green fluorescent protein expressing plasmid (pEGFP-N1). Vessels were electroporated with eight 10-ms pulses of 200 V/cm. Sixty seconds after electroporation, the DNA solution was removed, the intestine returned to the abdomen and the abdominal wall closed with suture and metal wound clips. Six hours to 5 days later, rats were sacrificed and electroporated vessels were recovered. Luciferase activity of the blood vessels was monitored. Gene expression was detected as early as 6 h postelectroporation, peaked at 1–3 days with levels up to 1 ng of reporter gene product per vessel segment and returned towards baseline by day 5. Histological analysis of blood vessel segments revealed green fluorescent protein-positive cells throughout the thickness of the vessel wall (endothelial cells to adventitia). Responses of electroporated vessels to vasoconstricting stimuli were indistinguishable from those of control vessels at either 2 or 40 days posttreatment. The results of this study provide evidence that electroporation is an effective means for introducing naked DNA into the blood vessel wall and form the basis for future studies on targeted gene therapy to the intact vasculature.


  

Author Contacts

Dr. David A. Dean
Department of Microbiology and Immunology
University of South Alabama College of Medicine, MSB 2112
Mobile, AL 36688 (USA)
Tel. +1 334 460 6406, Fax +1 334 460 7931, E-Mail dean@sungcg.usouthal.edu

  

Article Information

Received: Received: November 18, 1999
Accepted after revision: March 27, 2000
Number of Print Pages : 9
Number of Figures : 5, Number of Tables : 0, Number of References : 25

  

Publication Details

Journal of Vascular Research
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)

Vol. 37, No. 5, Year 2000 (Cover Date: September-October 2000)

Journal Editor: M.J. Mulvany, Aarhus
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/journals/jvr


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Published online: 10/2/2000

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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