Journal Mobile Options
Table of Contents
Vol. 19, No. 6, 1985
Issue release date: 1985
Caries Res 1985;19:490–496

Lesion Formation and Lesion Remineralization in Enamel under Constant Composition Conditions

A New Technique with Applications

Buskes J.A.K.M. · Christoffersen J. · Arends J.
aDental School, State University Groningen, The Netherlands; bMedicinsk-Kemisk Institut, Panum Institute, University of Copenhagen, Denmark

Individual Users: Register with Karger Login Information

Please create your User ID & Password

Contact Information

I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in


A new apparatus for the de- and remineralization of enamel in vitro under constant composition conditions is described. A de- or remineralizing solution flows over the specimens at a controlled rate. During artificial lesion formation (using an acetate buffer containing methylhydroxydiphosphonate, MHDP), the composition of the solution is constant. The Ca and phosphate variation is less than 3%, pH variation is less than 0.03 pH unit. For enamel these variations are within the biological variations between enamel specimens. Experimentally it was found that the liquid streaming over the enamel surface does not measurably remove material from the surface (erosion) with the experimental error of 0.1 μm. This was measured by following microhardness indentations during demineralization. The effect of various MHDP concentrations on demineralization was investigated in the range of 2–50 μM. Even low concentrations of MHDP prove to be effective in the microhardness increase and lesion depth reduction during demineralization. In the demineralization experiment it is shown by means of microradiography, hardness measurements as well as light microscopy that the method described quickly produces reproducible lesions. In a remineralization experiment of demineralized enamel effective remineralization of the lesions occurs in 360 h, giving a threefold increase in microhardness and a twofold decrease in lesion depth.

 goto top of outline Author Contacts

J.A.K.M. Buskes, Dental School, State University Groningen, 9713 AV Groningen (The Netherlands)

 goto top of outline Article Information

Published online: November 18, 2009
Number of Print Pages : 7

 goto top of outline Publication Details

Caries Research

Vol. 19, No. 6, Year 1985 (Cover Date: 1985)

Journal Editor: Shellis R.P. (Bristol)
ISSN: 0008-6568 (Print), eISSN: 1421-976X (Online)

For additional information:

Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50