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Table of Contents
Vol. 30, No. 5-6, 2009
Issue release date: December 2009
Section title: Research Article
Tumor Biol 2009;30:300–309
(DOI:10.1159/000261073)

Isolation and Characterization of Spheroid Cells from Human Malignant Melanoma Cell Line WM-266-4

Na Y.R.a · Seok S.H.b · Kim D.J.a · Han J.H.a · Kim T.H.a · Jung H.a · Lee B.H.c · Park J.H.a
aDepartment of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute and bInstitute for Experimental Animals, College of Medicine, Seoul National University, Seoul, and cDivision of Biological Resources Coordination, National Institute of Biological Resources, Environmental Research Complex, Incheon, Republic of Korea

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: May 18, 2009
Accepted: August 04, 2009
Published online: November 23, 2009
Issue release date: December 2009

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI

Abstract

Background/Aims: Spheroid cells which can grow as nonattached spheroids in vitro culture condition are considered as tumor-initiating cells that have properties similar to those of stem cells. However, the existence of spheroid cells in WM-266-4, a human malignant metastatic melanoma cell line, has not yet been reported. Methods: Accordingly, we investigated whether WM-266-4 can form spheroids, and characterized these spheroids using qRT-PCR, histology, immunohistochemistry and xenograft. Results: WM-266-4 contains a small subpopulation of cells that grow as spheroids and express genes strongly related to tumor malignancy and stem-like factors. Second, histological analysis of the spheres revealed that they consist of 300–400 round cells per sphere with a high karyoplasmic ratio. They have a basophilic cytoplasm and are highly pleomorphic in size, and sometimes multinucleated and giant. Third, although there were differences between the spheroid and bulk cells, they both have high tumorigenic potential, as both cell types formed a tumor mass upon injection of only 100 cells in nude mice. Conclusion: We characterized the spheroid cells in an established melanoma cell line. We suggest that enriched spheroid cells might contain more dedifferentiated progenitor cells, but we could not conclude spheroid cells are cancer stem cells.

© 2009 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: May 18, 2009
Accepted: August 04, 2009
Published online: November 23, 2009
Issue release date: December 2009

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI


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