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Table of Contents
Vol. 25, No. 2-3, 2010
Issue release date: January 2010
Section title: Original Paper
Cell Physiol Biochem 2010;25:241–252
(DOI:10.1159/000276558)

The Double Edge of Reactive Oxygen Species as Damaging and Signaling Molecules in HL60 Cell Culture

Ferrer M.D. · Sureda A. · Mestre A. · Tur J.A. · Pons A.
Laboratori de Ciències de l’Activitat Física, Departament de Biologia Fonamental i Ciències de la Salut. Grup de Nutrició Comunitaria i Estrés Oxidatiu, IUNICS, Universitat de les Illes Balears, Palma de Mallorca
email Corresponding Author

Dr. Antoni Pons Biescas

Laboratori de Ciències de l’Activitat Física

Universitat de les Illes Balears

Ctra. Valldemossa Km 7.5, E-07122 Palma de Mallorca, Illes Balears (Spain)

Tel. +34-971173171, Fax: +34-971173184, E-Mail antonipons@uib.es

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Abstract

Aims: Our aim was to establish the conditions in which reactive oxygen species produce pathological or hormetic effects on HL60 cells. Methods: HL60 cells were treated with either single bouts (1, 10 and 100 µM) or a sustained production (0.1, 1.0 and 10.0 nM/s) of H2O2. Results: Exposure to 10 and 100 µM H2O2 activated catalase, glutathione peroxidase and glutathione reductase through post-transcriptional mechanisms and induced oxidative modification of proteins. When cells where exposed to sustained H2O2 production, a clear dose-response effect was detected in the activity of the antioxidant enzymes catalase, glutathione peroxidase and Mn-SOD, with higher concentrations of H2O2 inducing greater enzyme activities. Catalase, HO-1, UCP-3, iNOS and PGC-1α expressions were activated after sustained exposure to 1 and 10 nM H2O2/s. Although the antioxidant defenses were activated, oxidative damage appeared in DNA and proteins in cells treated with 1 and 10 nM/s. Conclusions: HL60 cells respond to exposure to sustained levels of H2O2 in a dose-response manner to H2O2 concentration by activating the expression and activity of the antioxidant machinery, although the activation of the antioxidant defenses is not enough to avoid the appearance of oxidative damage. Of the two designs proposed, continuous exposure seems to be more appropriate to study the antioxidant response of HL60 cells to H2O2.

© 2010 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: October 22, 2009
Published online: January 12, 2010
Issue release date: January 2010

Number of Print Pages: 12
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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