External Qi of Yan Xin Qigong Induces Apoptosis and Inhibits Migration and Invasion of Estrogen-Independent Breast Cancer Cells Through Suppression of Akt/NF-ĸB SignalingYan X. · Shen H. · Jiang H. · Hu D. · Zhang C. · Wang J. · Wu X.
1Chongqing Institute of Traditional Chinese Medicine, Chongqing,2New Medical Science Research Institute, New York,3Brigham and Women’s Hospital and Harvard Medical School, Boston,4Dana-Farber Cancer Institute and Harvard Medical School, Boston,5McMaster University, Hamilton
The antitumor effects of external Qi of Yan Xin Qigong (YXQ-EQ) have been widely described over the past three decades. To gain a better understanding of the mechanisms underlying YXQ-EQ’s antitumor effects, in the present study we investigated its effects on growth, migration, invasion and apoptosis of breast cancer cells and the underlying molecular mechanisms. We show that YXQ-EQ treatment caused a time-dependent reduction in viability, blocked clonogenic growth and induced apoptosis in estrogen-independent breast cancer MDA-MB-231 cells. Furthermore, YXQ-EQ treatment blocked migration and invasion of MDA-MB-231 cells. Biochemically, YXQ-EQ treatment markedly inhibited constitutive and EGF-induced Akt phosphorylation. YXQ-EQ also substantially repressed NF-ĸB activity, resulting in decreased expression of anti-apoptotic Bcl-2, Bcl-XL, XIAP and survivin proteins. These findings suggest that YXQ-EQ may induce apoptosis and inhibition of migration and invasion of MDA-MB-231 cells through the repression of Akt/NF-ĸB signaling.
Dana-Farber Cancer Institute and Harvard Medical School
44 Binney Street, Boston, MA 02115 (USA)
Tel. +1 617 582 8303, Fax: +1 617 582 7992
Accepted: October 13, 2009
Published online: January 12, 2010
Number of Print Pages : 8
Cellular Physiology and Biochemistry (International Journal of Experimental Cellular Physiology, Biochemistry and Pharmacology)
Vol. 25, No. 2-3, Year 2010 (Cover Date: 2010)
Journal Editor: F. Lang, Tübingen
ISSN: 1015–8987 (Print), eISSN: 1421–9778 (Online)
For additional information: http://www.karger.com/journals/cpb