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Vol. 61, No. 1, 2000
Issue release date: July 2000
Section title: Original Paper
Pharmacology 2000;61:37–42
(DOI:10.1159/000028378)

M2 and M4 Muscarinic Receptor Subtypes Couple to Activation of Endothelial Nitric Oxide Synthase

Waid D.K. · Chell M. · El-Fakahany E.E.
Department of Psychiatry, University of Minnesota Medical School, Minneapolis, Minn., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/7/2000

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA

Abstract

In this report we studied coupling of M2 and M4 muscarinic acetylcholine receptors to activation of endothelial nitric oxide synthase (eNOS). Chinese hamster ovary cells that co-express the individual receptor subtypes and eNOS in a stable fashion were used as a model. Activation of eNOS was assayed by measuring increasing levels of cyclic GMP in admixed cells that contain guanylate cyclase. Activation of both M2 or M4 muscarinic receptors resulted in marked activation of eNOS, in a time- and concentration-dependent manner. The time course of the response exhibited a transient peak, followed by a sustained lower plateau. While the sustained phase was dependent on influx of extracellular calcium, the transient response showed dependency on both mobilization of intracellular calcium and extracellular influx.


  

Author Contacts

Esam E. El-Fakahany
Division of Neuroscience Research in Psychiatry
University of Minnesota Medical School, Box 392, Mayo Memorial Building
Minneapolis, MN 55455 (USA)
Tel. +1 612 624 8432, Fax +1 612 624 8935, E-Mail elfak001@maroon.tc.umn.edu

  

Article Information

Received: Received: August 5, 1999
Accepted: November 8, 1999
Number of Print Pages : 6
Number of Figures : 6, Number of Tables : 0, Number of References : 28

  

Publication Details

Pharmacology (International Journal of Experimental and Clinical Pharmacology)
Founded 1959 as ‘Medicina Experimentalis’, continued 1965–1967 as ‘Medicina et Pharmacologia Experimentalis’; Founder: R. Domenjoz (1959–1977); Successors: B.B. Brodie (1968–1977); K. Lederis (1973–1985)

Vol. 61, No. 1, Year 2000 (Cover Date: Released July 2000)

Journal Editor: J. Donnerer, Graz; E.S. Vesell, Hershey, Pa.; T. Watanabe, Sendai
ISSN: 0031–7012 (print), 1423–0313 (Online)

For additional information: http://www.karger.com/journals/pha


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/7/2000

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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