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Vol. 17, No. 5, 2010
Issue release date: May 2010
Section title: Original Paper
Neuroimmunomodulation 2010;17:305–313
(DOI:10.1159/000292022)

Cytokines Induce NF-ĸB, Nurr1 and Corticotropin-Releasing Factor Gene Transcription in Hypothalamic 4B Cells

Kageyama K.a · Kagaya S.a · Takayasu S.a · Hanada K.a · Iwasaki Y.b · Suda T.a
aDepartment of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki, and bDepartment of Endocrinology, Metabolism and Nephrology, Kochi Medical School, Kochi University, Nankoku, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/26/2009
Accepted: 9/18/2009
Published online: 4/17/2010
Issue release date: May 2010

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 0

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM

Abstract

Objective: In the hypothalamus, corticotropin-releasing factor (CRF) plays a central role in regulating stress responses. Cytokines are important mediators of the interaction between the neuroendocrine and immune systems, and are implicated in the regulation of CRF expression. Following inflammatory challenges, interleukin (IL)-1 or IL-6 stimulates the hypothalamic-pituitary-adrenal axis. CRF promoter contains multiple nuclear factor (NF)-ĸB and Nurr1 binding sites. In the present study, we determined the ability of the signaling pathways to activate the CRF gene in the hypothalamic paraventricular nucleus following inflammatory challenge. Methods: Cytokine-induced changes in CRF gene expression were examined in the hypothalamic system. Luciferase assay and Western blotting were performed to assess transcriptional activity and the nuclear translocation of transcriptional factors. Results: IL-1β, IL-6 and tumor necrosis factor (TNF)-α stimulated the nuclear expression levels of NF-ĸB, NF-ĸB-dependent Nurr1 and c-Fos proteins. Direct stimulatory effects of TNF-α and IL-1β, in addition to IL-6, were found on the transcriptional activity of the CRF gene in hypothalamic 4B cells. Conclusion: These cytokines are involved in the regulation of CRF gene activity in hypothalamic cells.

© 2010 S. Karger AG, Basel


  

Author Contacts

Kazunori Kageyama, MD, PhD
Department of Endocrinology and Metabolism
Hirosaki University Graduate School of Medicine, 5 Zaifu-cho
Hirosaki, Aomori 036-8562 (Japan)
Tel. +81 172 39 5062, Fax +81 172 39 5063, E-Mail kkageyama@hkg.odn.ne.jp

  

Article Information

Received: June 26, 2009
Accepted after revision: September 18, 2009
Published online: April 17, 2010
Number of Print Pages : 9
Number of Figures : 3, Number of Tables : 0, Number of References : 28

  

Publication Details

Neuroimmunomodulation

Vol. 17, No. 5, Year 2010 (Cover Date: May 2010)

Journal Editor: Savino W. (Rio de Janeiro)
ISSN: 1021-7401 (Print), eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/26/2009
Accepted: 9/18/2009
Published online: 4/17/2010
Issue release date: May 2010

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 0

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM


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