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Vol. 211, No. 3, 1997
Issue release date: 1997
Section title: Perspectives in Glaucoma Research
Ophthalmologica 1997;211:192–200
(DOI:10.1159/000310788)

Peroxide-Induced Damage in Lenses of Transgenic Mice with Deficient and Elevated Levels of Glutathione Peroxidase

Reddy V.N. · Lin L.R. · Ho Y.-S. · Magnenat J.-L. · Ibaraki N. · Giblin F.J. · Dang L.
aEye Research Institute, Oakland University, Rochester, Mich., and, bInstitute of Chemical Toxicology, Wayne State University, Detroit, Mich., USA

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Article / Publication Details

First-Page Preview
Abstract of Perspectives in Glaucoma Research

Published online: 4/1/2010

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH

Abstract

Transgenic mice with elevated glutathione peroxidase (GSHPx) activity and gene knockout animals with a deficiency of the enzyme were used to investigate the role of GSHPx in defending the lens against H2O2-induced damage. The effects of peroxide on cultured lenses were determined by using light and transmission electron microscopy to evaluate morphological changes occurring in the epithelium and superficial cortex of the central and equatorial regions of the lens. DNA single-strand breaks in the epithelium were also examined. Following a 30-min exposure to 25 μM H2O2, lenses from normal animals showed distinct changes in the morphology of both the epithelium and superficial cortex. The damage to these cells was extensive in lenses of gene knockout mice in which activity of GSHPx was undetectable. In marked contrast, lenses of transgenic mice, which had 5-fold higher activities of GSHPx, were able to resist the cyto-toxic effects. Similar to damage to cell morphology, the extent of DNA strand breaks was significantly lower (40% of control) in H2O2-exposed lenses as compared to normal lenses while DNA damage in gene knockout lenses was 5 times greater than that of GSHPx-rich transgenic lenses. The present studies extend our previous findings on the role of the glutathione redox cycle in the detoxification of peroxide and demonstrate that an increase in GSHPx activity protects the lens against peroxide-induced changes in cell morphology and DNA strand breaks.


  

Author Contacts

V. N. Reddy, Eye Research Institute, Oakland University, Rochester, MI 48309-4401 (USA)

  

Article Information

Published online: April 01, 2010
Number of Print Pages : 9

  

Publication Details

Ophthalmologica (International Journal of Ophthalmology)

Vol. 211, No. 3, Year 1997 (Cover Date: 1997)

Journal Editor: Cunha-Vaz J. (Coimbra)
ISSN: 0030-3755 (Print), eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH


Article / Publication Details

First-Page Preview
Abstract of Perspectives in Glaucoma Research

Published online: 4/1/2010

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH


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