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Vol. 153, No. 4, 2010
Issue release date: November 2010
Section title: Original Paper
Int Arch Allergy Immunol 2010;153:323–334
(DOI:10.1159/000316343)

Effect on Cell Surface Markers following Allergen-Induced Desensitization of Human Whole-Blood Basophils

Lund G. · Jacobi H. · Stahl Skov P. · Holm J. · Lund K.
aExperimental Immunology, ALK-Abelló; bReflab ApS and Allergycenter, Odense University Hospital, Hørsholm, Denmark

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/21/2009
Accepted: 1/20/2010
Published online: 6/17/2010

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: Allergen-specific immunotherapy (SIT) leads to reduced symptoms upon allergen exposure through as yet unresolved mechanisms. Desensitization of basophils to specific allergens during the updosing phase of injection immunotherapy may contribute to the clinical effect of SIT. Here we report a protocol for efficient in vitro allergen-mediated desensitization of basophils in whole blood and the effect of desensitization on the expression of basophil activation markers (CD203c and CD63) as well as histamine release in response to allergen challenge. Methods: Whole blood from grass pollen-allergic subjects was incubated with Phleum pratense extract by stepwise increase of the allergen concentration in the culture from well below to well above the allergen threshold concentration for activation of basophils. Desensitization was determined by measuring the expression of the basophil activation markers CD63 and CD203c by FACS following challenge with high allergen concentrations. Results: The basophil desensitization protocol reported here affected both the expression of the cell-surface markers and the levels of histamine release. Following the stepwise desensitization procedure the whole-blood basophils were not activated when challenged with more than 10-fold increased allergen concentration. Conclusion: We have established a protocol for basophil desensitization. By mimicking the updosing phase of immunotherapy we raised the allergen threshold for basophil activation and obtained efficient desensitization for all donors. We showed that conditions leading to desensitization affect histamine release and expression of different basophil markers alike.


  

Author Contacts

Correspondence to: Gitte Lund
Experimental Immunology, ALK-Abelló
Bøgealle 6-8, DK–2970 Hørsholm (Denmark)
Tel. +45 45 74 8103, Fax +45 45 74 8690, E-Mail gildk@alk-abello.com

  

Article Information

Received: July 21, 2009
Accepted after revision: January 20, 2010
Published online: June 17, 2010
Number of Print Pages : 12
Number of Figures : 6, Number of Tables : 1, Number of References : 35

  

Publication Details

International Archives of Allergy and Immunology

Vol. 153, No. 4, Year 2010 (Cover Date: November 2010)

Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/21/2009
Accepted: 1/20/2010
Published online: 6/17/2010

Number of Print Pages: 12
Number of Figures: 6
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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