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Efficacy of Chlorhexidine, Polihexanide and Tissue-Tolerable Plasma against Pseudomonas aeruginosa Biofilms Grown on Polystyrene and Silicone MaterialsHübner N.-O.a · Matthes R.a · Koban I.b · Rändler C.a · Müller G.a · Bender C.a · Kindel E.c · Kocher T.b · Kramer A.a
aInstitute of Hygiene and Environmental Medicine, and bCentre for Dental and Oral Medicine and Maxillofacial Surgery, Ernst Moritz Arndt University Greifswald, and cLeibniz Institute for Plasma Research and Technology, Greifswald, Germany Corresponding Author
Nils-Olaf Hübner, MD
Institute of Hygiene and Environmental Medicine
Ernst Moritz Arndt University Greifswald, Walther Rathenau Strasse 49a
DE–17489 Greifswald (Germany)
Tel. +49 383 451 5546, Fax +49 383 451 5541, E-Mail email@example.com
Background: The formation of biofilms is crucial in the pathogenesis of many acute and subacute microbial infections, including chronic wounds and foreign-body-related infections. Topical antimicrobial therapy with chemical antiseptics or physical treatment with tissue-tolerable plasma (TTP) may be promising to control bacterial infection. Methods: We assessed the efficacy of 0.1% chlorhexidine digluconate (CHX), 0.02 and 0.04% polihexanide (polyhexamethylene biguanide, PHMB) and of TTP against Pseudomonas aeruginosa SG81 biofilm grown in microtitre plates (polystyrene) and on silicone materials in an artificial wound fluid. Results: Overall, PHMB was as effective as CHX in reducing the total amount of biofilm (gentian violet assay) and in reducing the bacterial metabolism in biofilms (XTT assay). TTP also led to a significant reduction in colony-forming units. Conclusion: The antimicrobial activity of PHMB in biofilms is comparable to that of CHX. TTP could become an interesting physical alternative to chemical antisepsis in the future.
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