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Vol. 155, No. 2, 2011
Issue release date: May 2011
Section title: Original Paper
Int Arch Allergy Immunol 2011;155:167–179
(DOI:10.1159/000319193)

On the Occurrence of the Diabetes-Associated Antigen GAD 65 in Human Sera

Tilz G.P.a · Wiltgen M.b · Borkenstein M.c
aMedical University of Graz, and bInstitute for Medical Informatics, Statistics and Documentation, and cUniversity Clinic for Pediatrics, Medical University of Graz, Graz, Austria

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 3/19/2010
Accepted: 7/7/2010
Published online: 12/23/2010
Issue release date: May 2011

Number of Print Pages: 13
Number of Figures: 9
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: Glutamic acid decarboxylase (GAD 65) is involved as an antigen in diabetes mellitus type 1 and is generally considered to be located intracellularly in pancreas β-cells. In this study we demonstrate its appearance in 64 human sera samples representing a cross-section of a blood bank. Method: The proof of GAD 65 in sera was done using an enzyme-linked immunosorbent assay (ELISA) setup where it was detected by interaction with corresponding antibodies labeled with an enzyme. The enzyme catalyzes a substrate reaction, resulting in a change of color, that is used for the quantitative evaluation of the antigen-antibody interaction. Result: The measurements showed that GAD 65 exists in various amounts in the sera of blood donors, with an average concentration of 58.00 ng/ml. The correlation analysis of samples stored at –80°C and at room temperature demonstrates the stability of GAD 65 at room temperature. The correlation coefficient between the GAD 65 concentrations of samples stored at room temperature and of the same samples after 1 week shows that the molecule remains stable. Conclusion: Our results encourage us to propose antigen GAD 65, due to its frequency in human sera in different concentrations and its stability, as a biomarker for the early diagnosis of type 1 diabetes and related inflammations.

© 2010 S. Karger AG, Basel


  

Author Contacts

Correspondence to: Dr. Gernot P. Tilz
Medical University of Graz
Auenbruggerplatz 15
AT–8036 Graz (Austria)
Tel. +43 676 372 0778, Fax +43 316 385 3590, E-Mail gernot.tilz@meduni-graz.at

  

Article Information

Received: March 19, 2010
Accepted after revision: July 7, 2010
Published online: December 23, 2010
Number of Print Pages : 13
Number of Figures : 9, Number of Tables : 2, Number of References : 47

  

Publication Details

International Archives of Allergy and Immunology

Vol. 155, No. 2, Year 2011 (Cover Date: May 2011)

Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 3/19/2010
Accepted: 7/7/2010
Published online: 12/23/2010
Issue release date: May 2011

Number of Print Pages: 13
Number of Figures: 9
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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