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Vol. 117, No. 2, 2011
Issue release date: January 2011
Section title: Original Paper
Nephron Exp Nephrol 2011;117:e39–e46
(DOI:10.1159/000320049)

Expression of Novel Podocyte-Associated Proteins sult1b1 and ankrd25

Xu X.a · Patrakka J.a, b · Sistani L.a · Uhlen M.c · Jalanko H.e · Betsholtz C.a, d · Tryggvason K.a
aDivision of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, bDepartment of Renal Medicine, Karolinska University Hospital, cDepartment of Biotechnology, Royal Institute of Technology, and dDepartment of Medicine, Karolinska Institute, Stockholm, Sweden; eHospital for Children and Adolescence, University of Helsinki, Helsinki, Finland

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/27/2009
Accepted: 3/24/2010
Published online: 8/18/2010
Issue release date: January 2011

Number of Print Pages: 1
Number of Figures: 4
Number of Tables: 0

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE

Abstract

Background/Aims: Podocytes have a unique function in the renal ultrafiltration that is achieved by expressing proteins that are highly specific to podocytes. In this study, we identified two novel podocyte-associated proteins. Methods: The expression of sult1b1 and ankrd25 in mouse tissues was studied by RT-PCR. The protein expression was studied by generating polyclonal antibodies that were used in Western blotting and immunohistochemistry. Results: By RT-PCR we detected sult1b1 expression only in glomerular, liver and brain tissues. By immunohistochemistry, sult1b1 was detected in the kidney exclusively in the Golgi apparatus of the podocyte. No expression outside the glomerulus was observed in the kidney. The ankrd25 transcript was detected in most mouse tissues analyzed by RT-PCR. In the kidney, however, immunohistochemistry showed that this protein was expressed only by podocyte, mesangial, and smooth muscle cells. In podocytes, ankrd25 was localized to foot processes. Conclusions: Identification of these two novel glomerulus-associated proteins opens up possibilities to investigate their role in the renal filter physiology and diseases. We speculate that sult1b1 may be involved in the sulfonylation of podocyte protein podocalyxin, whereas ankrd25 may contribute to controlling actin dynamics in podocyte foot processes.

© 2010 S. Karger AG, Basel


  

Article Information

Received: July 27, 2009
Accepted: March 24, 2010
Published online: August 18, 2010
Number of Print Pages : 8
Number of Figures : 4, Number of Tables : 0, Number of References : 21

  

Publication Details

Nephron Experimental Nephrology

Vol. 117, No. 2, Year 2011 (Cover Date: January 2011)

Journal Editor: Hughes J. (Edinburgh)
ISSN: 1660-2129 (Print), eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/27/2009
Accepted: 3/24/2010
Published online: 8/18/2010
Issue release date: January 2011

Number of Print Pages: 1
Number of Figures: 4
Number of Tables: 0

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE


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