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Vol. 47, No. 1, 2012
Issue release date: December 2011
Section title: Original Paper
Ophthalmic Res 2012;47:39–46
(DOI:10.1159/000322801)

A Decrease in the Density of HLA-DR-Positive Cells Occurs Faster in Corneas Stored in Organ Culture than under Hypothermic Conditions

Al-Fakih A. · Faltus V. · Jirsova K.
aLaboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, and bOcular Tissue Bank, General Teaching Hospital and First Faculty of Medicine, Charles University in Prague, and cEuroMISE Center, Department of Medical Informatics, Institute of Computer Sciences AS CR, Prague, Czech Republic

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/5/2008
Accepted: 11/3/2010
Published online: 6/22/2011

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 3

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: http://www.karger.com/ORE

Abstract

Aims: To compare the number of antigen-presenting cells (APC) at various locations in fresh human corneoscleral disks and in those that are stored for grafting under hypothermic conditions or in organ culture (OC) with the aim of determining the conditions under which the decline in APC numbers is most substantial. Methods: Cryosections obtained from fresh corneoscleral disks and disks stored under hypothermic (Optisol-GS) or OC conditions were used. The density of HLA-DR-positive cells was determined on cross sections using enzyme immunohistochemistry (alkaline phosphatase-antialkaline phosphatase technique). Longitudinal sections were used for detecting ATPase activity. Results: The densities of HLA-DR-positive cells in both the epithelium and stroma increased from the central (3.79 and 0.61/mm2) to the peripheral cornea (5.56 and 1.35/mm2) as well as to the limbus and conjunctiva. A marked decrease in the number of HLA-DR-positive cells occurred after 7 days of storage in all corneal areas, the limbus and conjunctiva, compared to fresh tissue. No positive cells were found in the epithelium of corneas after 14 days in OC and after 21 days in hypothermic storage. Twenty-eight days of storage in OC led to the complete absence of HLA-DR-positive cells in the epithelium of the limbus and conjunctiva, and to a significant reduction in the stroma. Conclusion: Corneas stored in OC longer than 14 days are likely to be less immunogenic than corneas stored under hypothermic conditions, thus resulting in a possible positive effect on prolonging graft survival.


  

Author Contacts

Katerina Jirsova, MSc, PhD
Laboratory of the Biology and Pathology of the Eye and Ocular Tissue Bank
VFN, U nemocnice 2
CZ–128 08 Prague (Czech Republic)
Tel. +420 224 963 008, Fax +420 224 962 338, E-Mail katerina.jirsova@vfn.cz

  

Article Information

Received: November 5, 2008
Accepted after revision: November 3, 2010
Published online: June 22, 2011
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 3, Number of References : 27

  

Publication Details

Ophthalmic Research (Journal for Research in Experimental and Clinical Ophthalmology)

Vol. 47, No. 1, Year 2012 (Cover Date: December 2011)

Journal Editor: Pleyer U. (Berlin)
ISSN: 0030-3747 (Print), eISSN: 1423-0259 (Online)

For additional information: http://www.karger.com/ORE


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/5/2008
Accepted: 11/3/2010
Published online: 6/22/2011

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 3

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: http://www.karger.com/ORE


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