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Table of Contents
Vol. 27, No. 6, 2011
Issue release date: June 2011
Section title: Original Paper
Cell Physiol Biochem 2011;27:641–652
(DOI:10.1159/000330073)

Expression and Function of Fibroblast Growth Factor (FGF) 7 during Liver Regeneration

Tsai S.-M.1 · Wang W.-P.1,2
1Institute of Medical Sciences, Tzu Chi University, Hualien,2Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan
email Corresponding Author

Wen-Pin Wang

No.701, Section 3, Chung-Yang Road, Hualien (Taiwan 970)

Tel. +886-3-8565301 ext. 2665, Fax +886-3-8578386

E-Mail wpwang@mail.tcu.edu.tw

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Abstract

Background/Aim: Previous studies have shown that fibroblast growth factors (FGFs) are involved in the process of liver injury repair. Liver regeneration after partial hepatectomy (PH) is impaired in transgenic mice expressing dominant-negative FGFR2b in hepatocytes. Although FGF7, a ligand specifically bound to FGFR2b, is expressed by activated hepatic stellate cells (HSCs) in fibrotic livers, the expressions and functions of FGF7 and FGFR2b after PH remain unexplored. Therefore, this study sought to examine the potential role of FGF7 signaling during liver regeneration. Methods: We examined the expression of FGF7 and FGFR2b in normal and regenerating livers. Effects of FGF7 on hepatocytes were examined in vitro using primary hepatocyte culture with FGF7 recombinant protein and in vivo by hydrodynamic-based gene transfer method. Results: We found that FGF7 expression was increased according to the activation status of HSCs after PH. The receptor, FGFR2b, was also increased in hepatocytes during liver regeneration. In vitro treatment with FGF7 protein activated ERK1/2 and promoted proliferation of hepatocytes isolated from regenerating livers. In vivo overexpression of exogenous FGF7 could notably promote hepatic proliferation and activate MAPKs after PH. Conclusion: This study suggests a role for activated HSC-expressed FGF7 in stimulating FGF signaling pathways in hepatocytes and regulating liver regeneration.

© 2011 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: March 16, 2011
Published online: June 17, 2011
Issue release date: June 2011

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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