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Vol. 4, No. 4, 2011
Issue release date: November 2011
Section title: Original Paper
J Nutrigenet Nutrigenomics 2011;4:192–202
(DOI:10.1159/000330226)

Effects of a Supplementation of n-3 Polyunsaturated Fatty Acids with or without Fish Gelatin on Gene Expression in Peripheral Blood Mononuclear Cells in Obese, Insulin-Resistant Subjects

Rudkowska I. · Ponton A. · Jacques H. · Lavigne C. · Holub B.J. · Marette A. · Vohl M.-C.
aInstitute of Nutraceuticals and Functional Foods (INAF), Laval University, Quebec, Qué., bMcGill University and Génome Québec Innovation Centre, Montreal, Qué., cDepartment of Food Science and Nutrition, and dQuebec Heart and Lung Institute Research Centre, Laval University, Quebec, Qué., eDepartment of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ont., and fLaboratory of Endocrinology and Genomics, Laval University Hospital Research Center, Quebec, Qué., Canada

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/2/2010 7:58:48 PM
Accepted: 5/31/2011
Published online: 8/26/2011

Number of Print Pages: 11
Number of Figures: 3
Number of Tables: 0

ISSN: 1661-6499 (Print)
eISSN: 1661-6758 (Online)

For additional information: http://www.karger.com/JNN

Abstract

Aim: To investigate gene expression changes in peripheral blood mononuclear cells (PBMCs) following an n-3 polyunsaturated fatty acid (PUFA) and n-3 PUFA plus fish gelatin (+FG) supplementation. Methods: A transcriptome comparison of 8-week supplementation with n-3 PUFA and n-3 PUFA+FG was carried out in PBMCs of 16 obese insulin-resistant subjects. Results: Erythrocyte n-3 PUFA concentration increased and plasma triglycerides decreased significantly without altering inflammatory parameters after both supplementations. n-3 PUFA supplementation changed the expression of 805 genes, whereas n-3 PUFA+FG supplementation altered the expression of 184 genes. Three genes were commonly changed: fatty acid desaturase 1, free fatty acid receptor 3, and ectodysplasin. Pathway analyses indicate changes in gene expression via the nuclear receptor peroxisome proliferator-activated receptor α pathway after both supplementations. Further, the extent of modifications in the expression of genes implicated in the inflammatory pathways – the oxidative stress response mediated by nuclear factor (erythroid-derived 2)-like 2, nuclear transcription factor ĸB, oxidative stress, and hypoxia-inducible factor signaling – was different after each supplementation. Conclusion: Although n-3 PUFA and n-3 PUFA+FG supplementations have a distinct impact on gene expression levels, the consequences on biochemical parameters and metabolic pathways were comparable after both supplementations.


  

Author Contacts

Marie-Claude Vohl, PhD
Institute of Nutraceuticals and Functional Foods (INAF), Laval University
Pavillon des Services, bureau 2729K, 2440, boulevard Hochelaga
Québec, QC G1V 0A6 (Canada)
Tel. +1 418 656 2131, ext. 4676, E-Mail marie-claude.vohl@fsaa.ulaval.ca

  

Article Information

Received: December 2, 2010
Accepted: May 31, 2011
Published online: August 26, 2011
Number of Print Pages : 11
Number of Figures : 3, Number of Tables : 0, Number of References : 37
Additional supplementary material is available online - Number of Parts : 1

  

Publication Details

Journal of Nutrigenetics and Nutrigenomics

Vol. 4, No. 4, Year 2011 (Cover Date: November 2011)

Journal Editor: Pérusse L. (Quebec, Que.)
ISSN: 1661-6499 (Print), eISSN: 1661-6758 (Online)

For additional information: http://www.karger.com/JNN


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/2/2010 7:58:48 PM
Accepted: 5/31/2011
Published online: 8/26/2011

Number of Print Pages: 11
Number of Figures: 3
Number of Tables: 0

ISSN: 1661-6499 (Print)
eISSN: 1661-6758 (Online)

For additional information: http://www.karger.com/JNN


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