Catechin-Rich Grape Seed Extract Supplementation Attenuates Diet-Induced Obesity in C57BL/6J MiceOhyama K. · Furuta C. · Nogusa Y. · Nomura K. · Miwa T. · Suzuki K.
Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan
Background: Grape seed extracts (GSE) are known to present health benefits such as antioxidative and anti-obesity effects in animal models. The purpose of this research is to determine whether the specially manufactured GSE, catechin-rich GSE (CGSE), can protect against obesity induced by a high-fat diet (HFD) and to address the mechanism underlying this effect. Methods: The componential analysis of CGSE was performed using liquid chromatography/mass spectrometry. Oxygen consumption and the respiratory quotient were determined using 500 mg/kg CGSE administered orally for 3 days in 14- to 15-week-old male C57BL/6J mice. Nine-week-old male C57BL/6J mice were supplemented with 0.5 or 1% CGSE in a HFD for 12 weeks, and their body weight and food intake were monitored. Blood and tissue samples were collected and analyzed. Results: The main polyphenol components of CGSE were catechin and epicatechin. CGSE supplementation in the HFD-induced obesity model chronically suppressed the increase in body weight and the weight of fat pads. Furthermore, CGSE improved metabolic parameter abnormalities and upregulated the fatty acid oxidation-related genes in the liver. Conclusions: These findings suggest that CGSE contains monomeric catechins in high concentrations and ameliorates HFD-induced obesity in C57BL/6J mice.
© 2011 S. Karger AG, Basel
Institute for Innovation, Ajinomoto Co., Inc.
1-1, Suzuki-cho, Kawasaki-ku
Kawasaki-shi 210-8681 (Japan)
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Received: April 11, 2011
Accepted after revision: June 30, 2011
Published online: August 9, 2011
Number of Print Pages : 9
Number of Figures : 3, Number of Tables : 5, Number of References : 31
Annals of Nutrition and Metabolism (Journal of Nutrition, Metabolic Diseases and Dietetics)
Vol. 58, No. 3, Year 2011 (Cover Date: August 2011)
Journal Editor: Koletzko B. (Munich)
ISSN: 0250-6807 (Print), eISSN: 1421-9697 (Online)
For additional information: http://www.karger.com/ANM