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Table of Contents
Vol. 28, No. 2, 2011
Issue release date: August 2011
Section title: Original Paper
Cell Physiol Biochem 2011;28:339–346

Sphingosine but not Sphingosine-1-phosphate Stimulates Suicidal Erythrocyte Death

Qadri S.M.1 · Bauer J.1 · Zelenak C.1 · Mahmud H.1 · Kucherenko Y.1 · Lee S.H.2 · Ferlinz K.1,* · Lang F.1
1Department of Physiology, University of Tübingen, Tübingen2Yonsei University College of Medicine, Seoul,*current address: The Medicines Company Deutschland GmbH, Munich
email Corresponding Author

Prof. Dr. Florian Lang

Physiologisches Institut der Universität Tübingen

Gmelinstr. 5, 72076 Tübingen (Germany)

Tel. +49 7071 29 72194, Fax +49 7071 29 5618

E-Mail florian.lang@uni-tuebingen.de

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Sphingosine kinase 1 phosphorylates sphingosine, which is converted to ceramide by ceramide synthetase. Ceramide triggers eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure at the erythrocyte surface. Erythrocytes lack sphingosine phosphate-degrading enzymes and thus store large quantities of sphingosine phosphate. The present study explored the influence of sphingosine and sphingosine phosphate on eryptosis. [Ca2+]i, was estimated from Fluo3 fluorescence, cell volume from forward scatter and PS exposure from annexin V-binding in FACS analysis. Sphingosine (0.1 – 10 µM) but not sphingosine-1- phosphate (0.1 – 10 µM) increased [Ca2+]i, decreased cell volume and increased PS-exposure. The observations disclose sphingosine, but not sphingosine-1-phosphate, as a strong inducer of eryptosis.

© 2011 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: May 03, 2011
Published online: August 16, 2011
Issue release date: August 2011

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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