Orchiectomy Attenuates Kidney Fibrosis after Ureteral Obstruction by Reduction of Oxidative Stress in MiceCho M.H.a · Jung K.-J.b · Jang H.-S.b · Kim J.I.b · Park K.M.b
Departments of aPediatrics and bAnatomy, Kyungpook National University School of Medicine, Daegu, South Korea
Background/Aims: Men are generally more prone to chronic kidney disease and progression to end-stage renal disease than women. However, the underlying mechanisms remain unclear. In this study, we investigated the role of reactive oxygen species and testosterone in the progression of renal fibrosis in mice with unilateral ureteral obstruction (UUO). Methods: Mice were subjected to either orchiectomy or sham operation 14 days before either UUO or sham surgery. Harvesting of the kidney was performed 7 days after the UUO surgery to measure the production of reactive oxygen species and expression of antioxidants such as catalase, copper-zinc superoxide dismutase, and manganese superoxide dismutase, as well as fibrosis markers including α-smooth muscle actin (α-SMA) and collagen. Results: UUO resulted in increased expression of α-SMA and collagen deposition in the kidneys of both female and male mice. These increases were significantly greater in males than females. Orchiectomy significantly reduced increases in α-SMA expression and collagen deposition when compared with intact male. UUO increased the production of hydrogen peroxide and lipid peroxidation along with the decreases in expression of manganese superoxide dismutase, copper-zinc superoxide dismutase, and catalase. These changes induced by UUO were significantly attenuated by orchiectomy. Conclusion: Males are more susceptible to UUO-induced kidney fibrosis compared with females, and the higher susceptibility of males is obviated by orchiectomy along with reduction in oxidative stress.
© 2011 S. Karger AG, Basel
M.H.C. and K.-J.J. contributed equally to this work.
Received: September 27, 2011
Accepted: October 17, 2011
Published online: December 3, 2011
Number of Print Pages : 10
Number of Figures : 6, Number of Tables : 0, Number of References : 37
American Journal of Nephrology
Vol. 35, No. 1, Year 2012 (Cover Date: January 2012)
Journal Editor: Bakris G. (Chicago, Ill.)
ISSN: 0250-8095 (Print), eISSN: 1421-9670 (Online)
For additional information: http://www.karger.com/AJN