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Vol. 101, No. 4, 2012
Issue release date: June 2012
Section title: Original Paper
Neonatology 2012;101:241–246
(DOI:10.1159/000334655)

Diagnosis of Neonatal Sepsis by Broad-Range 16S Real-Time Polymerase Chain Reaction

Ohlin A.a · Bäckman A.b · Ewald U.d · Schollin J.c · Björkqvist M.a, c
aDepartment of Pediatrics, bClinical Research Centre, Örebro University Hospital and cHealth and Medical Sciences, Örebro University, Örebro, and dWomen’s and Children’s Health, Uppsala University, Uppsala, Sweden

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/16/2011
Accepted: 10/22/2011
Published online: 12/28/2011
Issue release date: June 2012

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 5

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO

Abstract

Background: The standard diagnostic test (blood culture) for suspected neonatal sepsis has limitations in sensitivity and specificity, and 16S polymerase chain reaction (PCR) has been suggested as a new diagnostic tool for neonatal sepsis. Objectives: To develop and evaluate a new real-time PCR method for detection of bacterial DNA in blood samples collected from infants with suspected neonatal sepsis. Methods: Immediately after blood culture, a study sample of 0.5–1.0 ml whole blood was collected and used for a novel 16S real-time PCR assay. All positive samples were sequenced. Detailed case studies were performed in all cases with conflicting results, to verify if PCR could detect pathogens in culture negative sepsis. Results: 368 samples from 317 infants were included. When compared with blood culture, the assay yielded a sensitivity of 79%, a specificity of 90%, a positive predictive value of 59%, and a negative predictive value of 96%. Seven of the 31 samples with a positive PCR result and a negative blood culture had definite or suspected bacterial sepsis. In five samples, PCR (but not blood culture) could detect a pathogen that was present in a blood culture collected more than 24 h prior to the PCR sample. Conclusions: This study presents an evaluation of a new real-time PCR technique that can detect culture-positive sepsis, and suggests that PCR has the potential to detect bacteria in culture-negative samples even after the initiation of intravenous antibiotics.

© 2011 S. Karger AG, Basel


  

Author Contacts

Andreas Ohlin, MD
Department of Pediatrics, Örebro University Hospital
SE–701 85 Örebro (Sweden)
Tel. +46 73 613 72 00
E-Mail andreas.ohlin@orebroll.se

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Article Information

Received: June 16, 2011
Accepted after revision: October 22, 2011
Published online: December 28, 2011
Number of Print Pages : 6
Number of Figures : 0, Number of Tables : 5, Number of References : 26
Additional supplementary material is available online - Number of Parts : 1

  

Publication Details

Neonatology (Fetal and Neonatal Research)

Vol. 101, No. 4, Year 2012 (Cover Date: June 2012)

Journal Editor: Halliday H.L. (Belfast), Speer C.P. (Würzburg)
ISSN: 1661-7800 (Print), eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/16/2011
Accepted: 10/22/2011
Published online: 12/28/2011
Issue release date: June 2012

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 5

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


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