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Table of Contents
39, No. 1, 2012
Issue release date: February 2012
Section title: Original Article · Originalarbeit
Free Access
Transfus Med Hemother 2012;39:29–35

Inactivation Effect of Standard and Fractionated Electron Beam Irradiation on Enveloped and Non-Enveloped Viruses in a Tendon Transplant Model

Schmidt T.a · Hoburg A.T.b · Gohs U.c · Schumann W.d · Sim-Brandenburg J.e · Nitsche A.e · Scheffler S.f · Pruss A.g
aJulius Wolff Institute, Center for Musculoskeletal Surgery, bSports Medicine & Arthroscopy Service, Department for Orthopedic Surgery and Traumatology, Charité – Universitätsmedizin Berlin, cLeibniz Institute of Polymer Research Dresden, dGamma-Service Produktbestrahlung GmbH, Radeberg, eRobert Koch-Institut, fChirurgisch-Orthopädischer Praxis Verbund (COPV), gUniversity Tissue Bank, Institute of Transfusion Medicine, Charité – Universitätsmedizin Berlin, Germany
email Corresponding Author

Prof. Dr. Axel Prus, University Tissue Bank, Institute of Transfusion Medicine, Charite – Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany, Tel. +49 30 450 5-52142, Fax -25976, axel.pruss@charite.de

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Background: For increasing allograft tendon safety in reconstructive surgery, an effective sterilization method achieving sterility assurance including viruses without impairing the grafts properties is needed. Fractionated Electron Beam (Ebeam) has shown promising in vitro results. The proof of sufficient virus inactivation is a central part of the process validation. Methods: The Ebeam irradiation of the investigated viruses was performed in an optimized manner (oxygen content < 0.1%, –78 °C). Using principles of a tendon model the virus inactivation kinetics for HIV-2, HAV, pseudorabies virus (PRV) and porcine parvovirus (PPV) were calculated as TCID50/ml and D10 value (kGy) for the fractionated (10 × 3.4 kGy) and the standard (1 × 34 kGy) Ebeam irradiation. Results: All viruses showed comparable D10 values for both Ebeam treatments. For sufficient virus titer reduction of 4 log10 TCID50/ml, a dose of 34 kGy of the fractionated Ebeam irradiation was necessary in case of HIV-2, which was the most resistant virus investigated in this study. Conclusion: The fractionated and the standard Ebeam irradiation procedure revealed comparable and sufficient virus inactivation capacities. In combination with the known good biomechanical properties of fractionated Ebeam irradiated tendons, this method could be a safe and effective option for the terminal sterilization of soft tissue allografts.

© 2012 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original Article &#x00B7; Originalarbeit

Received: October 10, 2011
Accepted: January 12, 2012
Published online: January 20, 2012
Issue release date: February 2012

ISSN: 1660-3796 (Print)
eISSN: 1660-3818 (Online)

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