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Table of Contents
29, No. 1-2, 2012
Issue release date: February 2012
Section title: Original
Cell Physiol Biochem 2012;29:163–170

Interaction of Diesel Exhaust Particles with Human, Rat and Mouse Erythrocytes in Vitro

Nemmar A.1 · Zia S.1 · Subramaniyan D.1 · Al-Amri I.2 · Al Kindi M.A.2 · Ali B.H.3
1Department of Physiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, UAE,2Department of Pathology, Electron Microscopy Unit, College of Medicine & Health Sciences, Sultan Qaboos University, Al-Khod, Sultanate of Oman,3Department of Pharmacology and Clinical Pharmacy, College of Medicine & Health Sciences, Sultan Qaboos University, Al-Khod, Sultanate of Oman
email Corresponding Author

Prof. A. Nemmar

Department of Physiology, Faculty of Medicine and Health Sciences

United Arab Emirates University, P.O. Box 17666, Al Ain (United Arab Emirates)

Tel. +971-37137533, Fax +971 3 7671966

E-Mail anemmar@uaeu.ac.ae or anemmar@hotmail.com

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Inhaled ultrafine (nano) particles can translocate into the bloodstream and interact with circulatory cells causing systemic and cardiovascular events. To gain more insight into this potential mechanism, we studied the interaction of diesel exhaust particles (DEP) with human, rat and mouse erythrocytes in vitro. Incubation of erythrocytes with DEP (1, 10 or 100 µg/ml) for 30 min caused the highest hemolytic effect (up to 38%) in rats, compared to small but significant hemolysis in mice (up to 2.5%) and humans (up to 0.7%). Transmission electron microscopy of erythrocytes revealed the presence of variable degrees of ultrafine (nano)-sized aggregates of DEP either internalized and/or adsorbed onto the erythrocytes in the three species. A significant amount of DEP was found in rat and mouse (but not human) erythrocytes. Lipid erythrocyte susceptibility to in vitro peroxidation measured by malondialdehyde showed a significant and dose-dependent increase in erythrocytes of rats, but not humans or mice. Unlike in human erythrocytes, total antioxidant status (TAS) and superoxide dismutase (SOD) activity in rats were significantly and dose- dependently decreased. In mouse erythrocytes, DEP caused a decreased in SOD (at 10 µg/ml) and TAS (at 100 µg/ml) activities. In conclusion, DEP caused species–dependent erythrocyte hemolysis and oxidative stress, and were either taken up and/or adsorbed onto the red blood cells. Rat (and to a lesser degree mouse) erythrocytes were susceptible to DEP. Human erythrocytes showed the highest resistance to the observed effects. These species difference should be noted when using rats and mice blood as models for humans.

© 2012 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original

Accepted: October 18, 2011
Published online: March 01, 2012
Issue release date: February 2012

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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