For Manuscript Submission, Check or Review Login please go to Submission Websites List.
For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.
Ubiquitin E3 Ligase A20 Contributes to Maintaining Epithelial Barrier FunctionHuang P.a · Geng X.-R.b · Yang G.b · Chen C.a · Liu Z.a · Yang P.-C.b
aDepartment of Gastroenterology, the Shanghai Tenth People‘s Hospital, Tongji University, Shanghai bDepartment of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada Corresponding Author
Dr. Pingchang Yang or Dr. Zhanju Liu
Room T3303, 50 Charlton Ave East, Hamilton, ON (Canada) L8N 4A6
Tel. +1905-522-1155, ext. 32934, E-Mail email@example.com
or 301 Yanchang Middle Road, Zhabei, Shanghai (China)
Tel. +86 2166301164, E-Mail firstname.lastname@example.org
Background and Aims: Epithelial barrier dysfunction is involved in the pathogenesis of allergic diseases; the mechanism is to be further understood. Ubiquitin E3 ligase A20 (A20) plays a role in maintaining the homeostasis in the body. This study aims to investigate the role of A20 in maintaining the epithelial barrier function. Methods: Human intestinal epithelial cell line, Caco-2 cells, was cultured to monolayers to test the endocytosis and degradation of a model allergen, ovalbumin (OVA). The role of A20 in the endosome/lysosome fusion in epithelial cells was tested with A20-sufficient and A20-deficient Caco-2 cells and visualized by immunocytochemistry. Results: Caco-2 cells could endocytose exogenous allergens (OVA) in culture. The endocytic OVA was degraded in A20-sufficient Caco-2 cells via the mechanism of endosome/lysosome fusion, while the A20-deficient Caco-2 monolayers converted the OVA to the basal compartment of transwells, which conserved the antigenicity reflected by that it induced T cell proliferation in an allergen-specific manner. A20 was required in the fusion of endosomes and lysosomes. Conclusion: A20 contributes to maintaining the epithelial barrier function.
© 2012 S. Karger AG, Basel