Relationship between the Expression of the Extracellular Matrix Genes SPARC, SPP1, FN1, ITGA5 and ITGAV and Clinicopathological Parameters of Tumor Progression and Colorectal Cancer DisseminationViana L.S. · Affonso Jr. R.J. · Silva S.R.M. · Denadai M.V.A. · Matos D. · Salinas de Souza C. · Waisberg J.
aPostgraduate Program in Interdisciplinary Surgery and bGenetics Laboratory (GRAACC), Federal University of São Paulo, São Paulo, and cBarretos Cancer Hospital, Fundação Pio XII, Barretos, Brazil
Objective: To evaluate the relationship between the expression of the extracellular matrix (ECM) genes SPARC, SPP1, FN1, ITGA5 and ITGAV and the histopathologic parameters of neoplastic progression and colorectal carcinoma (CRC) dissemination. Methods: A retrospective study was conducted in 114 patients with stage I–IV CRC who underwent primary tumor resection. Quantitative real-time PCR and immunohistochemistry (IHC) assays were performed in samples obtained from the primary tumors. The correlation between the expression of these markers and the expression of p53, Bcl-2, Ki67, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor was assessed with the Spearman coefficient (r). Results: The ITGAV gene was found to be significantly amplified in tumors with positive perineural invasion (p = 0.028). Expression of the SPARC, SPP1, FN1, ITGA5 and ITGAV genes did not correlate with TNM staging. A direct relationship between ITGAV and EGFR expression (r = 0.774; p < 0.001) was observed by IHC. Conclusions: ECM gene expression did not correlate with classical prognostic factors for CRC, but overexpression of the ITGAV gene and protein was correlated with an increased risk of perineural invasion. The relationship between ITGAV and EGFR expression suggests the possibility of crosstalk in this signal pathway.
Luciano de Souza Viana
Avenida Lins, número 90, Apt 22
Barretos, SP 14784-173 (Brazil)
Received: March 23, 2012
Accepted after revision: September 11, 2012
Published online: October 31, 2012
Number of Print Pages : 11
Number of Figures : 1, Number of Tables : 5, Number of References : 50
Oncology (International Journal for Cancer Research and Treatment)
Vol. 84, No. 2, Year 2013 (Cover Date: December 2012)
Journal Editor: Markman M. (Philadelphia, Pa.)
ISSN: 0030-2414 (Print), eISSN: 1423-0232 (Online)
For additional information: http://www.karger.com/OCL