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Vol. 102, No. 2, 1999
Issue release date: October 1999
Section title: Original Paper
Acta Haematol 1999;102:66–71
(DOI:10.1159/000040972)

The Polysaccharide, PGG-Glucan, Enhances Human Myelopoiesis by Direct Action Independent of and Additive to Early-Acting Cytokines

Turnbull J.L. · Patchen M.L. · Scadden D.T.
aMassachusetts General Hospital, Harvard Medical School, Boston, Mass., and bAlpha-Beta Technology, Worcester, Mass., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 10/6/1999

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 1

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: http://www.karger.com/AHA

Abstract

β-Glucans stimulate leukocyte anti-infective activity, enhance murine hematopoietic recovery following bone marrow injury and mobilize murine progenitor cells from bone marrow. This study evaluated the in vitro hematopoietic potential of the β-glucan, PGG-glucan, on human bone marrow mononuclear cells (BMMC) and CD34+ BMMC compared with protein cytokines. In the presence of submaximal concentrations of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF; 0.5 ng/ml), PGG-glucan significantly increased BMMC myeloid colony formation comparable to the increase observed with either interleukin-3 (rhIL-3) or stem cell factor (rhSCF). Moreover, the addition of PGG-glucan to cultures containing GM-CSF + IL-3 or GM-CSF + SCF significantly augmented granulocyte-macrophage colony production above baseline, demonstrating that PGG-glucan acts independently of those early-acting cytokines and can enhance their activity in an additive manner. Anti-PGG-glucan monoclonal antibody specifically abrogated the growth-enhancing effect of added PGG-glucan in a saturable manner and other control carbohydrate polymers failed to affect colony formation. Further, PGG-glucan was not associated with induction of IL-6, GM-CSF production and removal of accessory cells by CD34+ cell isolation did not alter the PGG-glucan effect. These data demonstrate that PGG-glucan acts on committed myeloid progenitors to enhance human hematopoietic activity by a mechanism of direct action independent of IL-3 or SCF and independent of secondary cytokine stimulation.


  

Author Contacts

David T. Scadden, MD
Massachusetts General Hospital, 13th Street
Building 149, Room 5212D, Boston, MA 02129 (USA)
Tel. +1 617 726 8166, Fax +1 617 726 4691
E-Mail scadden.david@mgh.harvard.edu

  

Article Information

Received: Received: October 22, 1998
Accepted: April 10, 1999
Number of Print Pages : 6
Number of Figures : 6, Number of Tables : 1, Number of References : 23

  

Publication Details

Acta Haematologica
Founded 1948
Affiliated with Molecular Biology of Hematopoiesis Symposium

Vol. 102, No. 2, Year 1999 (Cover Date: Released October 1999)

Journal Editor: I. Ben-Bassat, Tel Hashomer
ISSN: 0001–5792 (print), 1421–9662 (Online)

For additional information: http://www.karger.com/journals/aha


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 10/6/1999

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 1

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: http://www.karger.com/AHA


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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