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Vol. 169, No. 1, 2001
Issue release date: 2001
Section title: Original Paper
Cells Tissues Organs 2001;169:12–20
(DOI:10.1159/000047856)

The Dynamic in vivo Distribution of Bone Marrow-Derived Mesenchymal Stem Cells after Infusion

Gao J. · Dennis J.E. · Muzic R.F. · Lundberg M. · Caplan A.I.
aSkeletal Research Center, Department of Biology and bDepartment of Radiology, Case Western Reserve University, Cleveland, Ohio, USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 4/27/2001

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) have the potential to differentiate along different mesenchymal lineages including those forming bone, cartilage, tendon, fat, muscle and marrow stroma that supports hematopoiesis. This differentiation potential makes MSCs candidates for cell-based therapeutic strategies for mesenchymal tissue injuries and for hematopoietic disorders by both local and systemic application. In the present study, rat marrow-derived MSCs were ex vivo culture-expanded, labeled with 111In-oxine, and infused into syngeneic rats via intra-artery (i.a.), intravenous (i.v.) and intraperitoneal cavity (i.p.) infusions. In addition, for i.a. and i.v. infusions, a vasodilator, sodium nitroprusside, was administered prior to the cell infusion and examined for its effect on MSC circulation. The dynamic distribution of infused MSCs was monitored by real-time imaging using a gamma camera immediately after infusion and at 48 h postinfusion. After 48 h, radioactivity in excised organs, including liver, lungs, kidneys, spleen and long bones, was measured in a gamma well counter and expressed as a percentage of injected doses. After both i.a. and i.v. infusion, radioactivity associated with MSCs was detected primarily in the lungs and then secondarily in the liver and other organs. When sodium nitroprusside was used, more labeled MSCs cleared the lungs resulting in a larger proportion detected in the liver. Most importantly, the homing of labeled MSCs to the marrow of long bones was significantly increased by the pretreatment with vasodilator. These results indicate multiple homing sites for injected MSCs and that the distribution of MSCs can be influenced by administration of vasodilator.


  

Author Contacts

Dr. Arnold I. Caplan
Skeletal Research Center, Department of Biology
Case Western Reserve University
2080 Adelbert Road, Cleveland, OH 44106-7080 (USA)
Tel. +1 216 368 3562, Fax +1 216 368 4077

  

Article Information

Accepted after revision: July 25, 2000
Number of Print Pages : 9
Number of Figures : 6, Number of Tables : 1, Number of References : 47

  

Publication Details

Cells Tissues Organs (in vivo, in vitro)
Founded 1945 as Acta Anatomica

Vol. 169, No. 1, Year 2001 (Cover Date: 2001)

Formerly Acta Anatomica

Journal Editor: H.-W. Denker, Essen; A.W. English, Atlanta, Ga.
ISSN: 1422–6405 (print), 1422–6421 (Online)

For additional information: http://www.karger.com/journals/cto


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 4/27/2001

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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