The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISHStrefford J.C. · Foot N.J. · Chaplin T. · Neat M.J. · Oliver R.T.D. · Young B.D. · Jones L.K.
aICRF Medical Oncology Unit, Queen Mary and Westfield College, London; bDepartment of Medical Oncology and cThe Orchid Cancer Appeal, St Bartholomew’s Hospital, London (UK)
The cell line U937, which has been used extensively for studies of myeloid differentiation, bears the t(10;11)(p13;q14) translocation which results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia [trithorax, Drosophila, homolog]; translocated to 10; alias AF10) gene and the Ap-3-like clathrin assembly protein, PICALM (Clathrin assembly lymphoid myeloid leukaemia). Apart from this translocation, very little is known about the other genetic alterations in this cell line that may represent significant events in disease progression. In this study, conventional G-banding, CGH and M-FISH have been used to characterise fully all of the cytogenetic alterations present in the U937 cell line. M-FISH analysis confirmed the presence of the t(10;11) and an apparently normal copy of both chromosomes 10 and 11. A t(1;5) translocation was observed as well as several unbalanced rearrangements. CGH detected amplifications resulting from duplications of 2q, 6p and 13q. These changes could result in fusion gene products involved in carcinogenesis or the positions of putative oncogenes and tumour suppressor genes. A good correlation between conventional G-banding, CGH and M-FISH was observed.
Request reprints from Jon C. Strefford, ICRF Medical Oncology Unit, Queen Mary and Westfield College, Charterhouse Square, London, EC1M 6BQ (UK); telephone: 0207 882 6003; fax: 0207 882 6004; email: J.Strefford@icrf.icnet.uk
Supported by the Orchid Cancer Appeal and by the Imperial Cancer Research Fund.
Received: Received 10 January 2001;
accepted 10 May 2001.
Number of Print Pages : 6
Number of Figures : 3, Number of Tables : 2, Number of References : 29
Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger
Vol. 94, No. 1-2, Year 2001 (Cover Date: 2001)
Journal Editor: H.P. Klinger, Bronx, N.Y.; M. Schmid, Würzburg
ISSN: 0301–0171 (print), 1422–9816 (Online)
For additional information: http://www.karger.com/journals/ccg