Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2Ward E.G. · Grosios K. · Markham A.F. · Jones P.F.
Molecular Medicine Unit, University of Leeds, St James’s University Hospital, Leeds (UK)
The family of human angiopoietins comprises factors with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to the endothelial cell-specific receptor, Tie2. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. In this study the genomic structures of all three human angiopoietins were characterised by direct sequencing of human genomic DNA from the appropriate P1 artificial chromosome (PAC) clones. The exact positions at the intron/exon boundaries and the lengths of all eight introns were determined. As would be expected from the homology of these three proteins, the positions of the introns in the three genes are highly conserved. The putative RNA transcription start site for each angiopoietin gene was also determined. Intron-specific primers were used to amplify each exon of angiopoietin-1 and angiopoietin-2 from individual genomic DNAs. Although no polymorphism has been detected in the coding region of angiopoietin-1, three independent polymorphisms have been identified for angiopoietin-2.
© 2002 S. Karger AG, Basel
Request reprints from Pamela F. Jones, Molecular Medicine Unit,University of Leeds, Clinical Sciences Building,St James’s University Hospital, Leeds, LS9 7TF (UK);telephone: 44 0113 2066099; fax: 44 0113 2444475;e-mail: email@example.com
Supported by the Yorkshire Cancer Research, British Heart Foundation, Wellcome Trust and Medical Research Council. We thank the St. James’s University Hospital Diabetes Foundation and in particular Mr. L. Silver for financial support for E.G.W. and Dr. S. Gilbey for his enthusiastic support.
Received: Received 19 December 2000;
revision accepted 24 September 2001.
Number of Print Pages : 8
Number of Figures : 4, Number of Tables : 5, Number of References : 29
Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger
Vol. 94, No. 3-4, Year 2001 (Cover Date: 2001)
Journal Editor: H.P. Klinger, Bronx, N.Y.; M. Schmid, Würzburg
ISSN: 0301–0171 (print), 1422–9816 (Online)
For additional information: http://www.karger.com/journals/ccg