Dilated Intercellular Spaces as Markers of Reflux Disease: Histology, Semiquantitative Score and Morphometry upon Light MicroscopyVillanacci V. · Grigolato P.G. · Cestari R. · Missale G. · Cengia G. · Klersy C. · Rindi G.
aPathology Service 2, bDepartment of Surgery (Digestive Endoscopy), University of Brescia and Spedali Civili, Brescia, and cBiometric and Clinical Epidemiology Service, IRCCS Policlinico San Matteo, Pavia, Italy
Background and Aims: A recent electron microscopy study suggested that dilated intercellular spaces (DIS) are specific for acid reflux-damaged esophageal epithelium. Electron microscopy is, however, expensive and difficult to apply to routine biopsies. The aims of this study are to establish a method for assessing DIS on light microscopy of esophageal biopsies and to estimate its association with current clinicopathological parameters of esophagitis. Materials and Methods: 21 patients with reflux symptoms were investigated. Light microscopy biopsies were assessed for DIS size by a semiquantitative method and computer-assisted, static morphometry. A DIS score accounting for DIS size and distribution was assigned to each patient and its association with 30 clinicopathological variables investigated by univariate and multivariate logistic regression. Results: Both the semiquantitative method and static morphometry identified 4 different classes of DIS size. The DIS score was significantly and independently associated with the esophageal symptoms score, the histological score of esophagitis and the relevant morphometry data. Conclusions: DIS may be efficiently assessed during light microscopy of routine esophageal biopsies. Since correlation with both the histology and the symptoms of esophagitis, the DIS score may be considered a novel parameter of esophagitis and is suggested for the routine evaluation of esophageal biopsies in patients with reflux disease.
Guido Rindi MD, PhD
Anatomia Patologica 2
Piazza Spedali Civili, 1
I–25124 Brescia (Italy)
Tel. +39 030 3995 830, Fax +39 030 3995 053, E-Mail email@example.com
Supported by the University of Brescia (G.R. and P.G.G.) and COFIN 99 (G.R.).
Received: Received: October 19, 2000
Accepted: January 23, 2001
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 3, Number of References : 17
Digestion (International Journal of Gastroenterology)
Founded as Archiv für Verdauungskrankheiten, 1895; continued as Gastroenterologia, 1939-1967
Vol. 64, No. 1, Year 2001 (Cover Date: 2001)
Journal Editor: R. Arnold, Marburg
ISSN: 0012–2823 (print), 1421–9867 (Online)
For additional information: http://www.karger.com/journals/dig