Journal Mobile Options
Table of Contents
Vol. 124, No. 1-3, 2001
Issue release date: January–March 2001
Section title: Immunotherapy and Pharmacotherapy
Int Arch Allergy Immunol 2001;124:406–410
(DOI:10.1159/000053772)

The Influence of CpG Motifs on a Protein or DNA-Based Th2-Type Immune Response against Major Pollen Allergens Bet v 1a, Phl p 2 and Escherichia coli-Derived β-Galactosidase

Hochreiter R.a · Hartl A.a · Freund J.a · Valenta R.b · Ferreira F.c · Thalhamer J.a
aInstitute of Chemistry and Biochemistry, University of Salzburg, bDepartment of Pathophysiology, University of Vienna, cInstitute of Genetics and General Biology, University of Salzburg, Austria

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Immunotherapy and Pharmacotherapy

Published online: 1/24/2012
Issue release date: January–March 2001

Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: DNA immunization and protein immunization with CpG motifs as adjuvants represent promising approaches in allergen-specific immunotherapy. Objective: We investigated the effect of coinjection or prepriming with CpG-ODN on Th2-type responses induced by gene gun and protein immunization. Methods: BALB/c mice were immunized with the gene gun using plasmid DNA containing the cDNAs coding for the genes of Bet v 1a, Phl p 2 and β-galactosidase or with the purified Al(OH)3-adsorbed proteins. In addition, CpG-ODN were applied by coinjection or by prepriming treatment. Antibody and cytokine responses were measured by ELISA, proliferative and cytotoxic responses were determined by standard labeling procedures. Furthermore, the allergenic activity of sera was measured by passive cutaneous anaphylaxis. Results: Gene gun immunization and protein immunization induced a clear Th2-type response for all antigens. The Th1-promoting effect of CpG-ODN coinjection together with gene gun immunization was restricted to β-galactosidase as indicated by the increase of IgG2a and a marked expression of IFN-γ. CpG motifs also increased the specific cytotoxic response against β-galactosidase. Prepriming with CpG-ODN and gene gun or protein immunization with Bet v 1a exhibited no significant difference to the non-CpG control group. However, sera from mice preprimed with CpG-ODN induced no anaphylaxis with gene gun immunization, but with protein immunization. Conclusions: The effect of CpG motifs in vivo depends on a variety of parameters like the nature of the antigen and the immunization modality. Furthermore, our studies indicate that a combination of CpG + DNA immunization may be more effective in antagonizing Th2 responses than the combination of CpG + protein immunization.

© 2001 S. Karger AG, Basel


  

Author Contacts

Correspondence to: Dr. Josef Thalhamer
Institute of Chemistry and Biochemistry, University of Salzburg
Hellbrunnerstrasse 34
A–5020 Salzburg (Austria)
Tel. +43 662 8044 5737, Fax +43 662 8044 5751, E-Mail Josef.Thalhamer@sbg.ac.at

  

Article Information

Number of Print Pages : 5
Number of Figures : 1, Number of Tables : 2, Number of References : 17

  

Publication Details

International Archives of Allergy and Immunology
Founded 1950

Vol. 124, No. 1-3, Year 2001 (Cover Date: January-March 2001)

Journal Editor: D. Kraft, Vienna
ISSN: 1018–2438 (print), 1423–0097 (Online)

For additional information:http://www.karger.com/journals/iaa


Article / Publication Details

First-Page Preview
Abstract of Immunotherapy and Pharmacotherapy

Published online: 1/24/2012
Issue release date: January–March 2001

Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 2

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.