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Table of Contents
Vol. 69, No. 4, 2001
Issue release date: May 2002
Section title: Original Paper
Pathobiology 2001;69:219–224
(DOI:10.1159/000055946)

Expression of MRE11 Complex (MRE11, RAD50, NBS1) and hRap1 and Its Relation with Telomere Regulation, Telomerase Activity in Human Gastric Carcinomas

Matsutani N.a,b · Yokozaki H.a · Tahara E.c · Tahara H.c · Kuniyasu H.d · Kitadai Y.b · Haruma K.e · Chayama K.b · Tahara E.f · Yasui W.a
aFirst Department of Pathology, bFirst Department of Internal Medicine and cDepartment of Cellular and Molecular Biology, Hiroshima University School of Medicine, Minami-ku, Hiroshima, dDepartment of Oncological Pathology Cancer Center, Nara Medical University, Kashihara, Nara, eDivision of Gastroenterology, Second Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Okayama, and fRadiation Effects Research Fundation, Minami-ku, Hiroshima, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 10, 2002
Issue release date: May 2002

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 4

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT

Abstract

The MRE11 complex (MRE11, RAD50, NBS1) are required for the repair of DNA double-strand breaks and have another important function in regulating telomere length. The silent information regulator (Sir) proteins required for telomere position effect also bind telomeres. hRap1 protein is a human ortholog of yeast Rap1 which regulates telomere length by interacting with TRF2 and is recruited to telomeres by TRF2. We examined the expression of the MRE11 complex (MRE11, RAD50, NBS1), Sir2 and hRAP1 in 20 gastric carcinomas by reverse transcription polymerase chain reaction and then analyzed the relation between telomerase activity and other telomerase components such as human telomerase reverse transcriptase (TERT), human telomerase RNA component (hTR), human telomerase-associated protein (TEP1), telomeric repeat binding factor 1 (TRF1), TRF2- and TRF1-interacting, ankyrin-related ADP-ribose polymerase (tankyrase) as well as TRF1-interacting nuclear protein 2 (TIN2). Of twenty gastric carcinomas examined, 13 (65%), 14 (70%), 16 (80%), 12 (60%) and 13 (65%) expressed MRE11, RAD50, NBS1, Sir2 and hRap1 at higher levels than corresponding nonneoplastic gastric mucosa, respectively. No obvious correlation was observed between MRE11 complex expression and telomerase activity or expression of TERT, hTR, TEP1, tankyrase and TIN2. Carcinomas with high TRF1 expression expressed significantly higher levels of MRE11 and RAD50 than those with low TRF1 expression (p < 0.05). On the other hand, carcinomas with high TRF2 expression expressed significantly higher levels of MRE11, NBS1 and hRap1 than those with low TRF2 expression (p < 0.05). These results suggest that gastric carcinomas with high TRF1 and TRF2 expression may need a large quantity of the MRE11 complex. Moreover, gastric carcinomas with high TRF1 expression may require a large quantity of hRap1.

© 2002 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 10, 2002
Issue release date: May 2002

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 4

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT


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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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