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Vol. 66, No. 4, 2001
Issue release date: May 2001
Section title: Original Paper
Urol Int 2001;66:205–211
(DOI:10.1159/000056616)

Intracellular Electrical Activity in Human Urinary Bladder Smooth Muscle: The Effect of High Sucrose Medium

Visser A.J. · van Mastrigt R.
Department of Urology-Urodynamics, Erasmus University Rotterdam, The Netherlands

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 5/17/2001

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 1

ISSN: 0042-1138 (Print)
eISSN: 1423-0399 (Online)

For additional information: http://www.karger.com/UIN

Abstract

Introduction: The primary key to pharmacotherapy of bladder instability is in the excitation-contraction coupling of detrusor smooth muscle cells. To study this process, simultaneous recordings of mechanical and electrical activity are required. However, recording of mechanical activity induces movement, which may affect the quality of intracellular recordings. Materials and Methods: We therefore compared the electrical activity of human detrusor smooth muscle cells in normal Krebs’ solution and in a hypertonic solution, which immobilizes the tissue, enabling us to study the effect of movement on the membrane potential. Carbachol and KCl were applied to induce contractions. Results: Sucrose in the medium made the tissue rigid and abolished its movement, while the electrical response was not affected. When compared with recordings in normal Krebs’ solution, the average resting membrane potential was not altered. However, the membrane potential was more stable, with far less spike-shaped potentials. The spike-shaped potential amplitude was larger, while the duration was decreased. Conclusions: Impairing the ability of tissue movement resulted in changes in the electrophysiological properties of detrusor smooth muscle cells. The results suggest that stretch has an effect on L-type Ca2+ channels.


  

Author Contacts

Anna J. Visser
Department of Urology-Urodynamics
Room EE 16-30, Erasmus University Rotterdam
NL–3000 DR Rotterdam (The Netherlands)
Tel. +31 10 408 7377, Fax +31 10 408 9451, E-Mail vanmastrigt@udn.fgg.eur.nl

  

Article Information

Received: Received: August 8, 2000
Accepted: November 23, 2000
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 1, Number of References : 30

  

Publication Details

Urologia Internationalis
Founded 1955 by G. Peter A. Narath and Heinrich Heusser; Continued 1968–1987 by G. Mayor

Vol. 66, No. 4, Year 2001 (Cover Date: Released May 2001)

Journal Editor: D. Hauri, Zürich; M.P. Wirth, Dresden
ISSN: 0042–1138 (print), 1423–0399 (Online)

For additional information: http://www.karger.com/journals/uin


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 5/17/2001

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 1

ISSN: 0042-1138 (Print)
eISSN: 1423-0399 (Online)

For additional information: http://www.karger.com/UIN


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