Expression of a candidate gene for the gonadoblastoma locus in gonadoblastoma and testicular seminomaLau Y.-F.C. · Chou P.M. · Iezzoni J.C. · Alonzo J.A. · Kömüves L.G.
Departments of aMedicine and dAnatomic Pathology, VA Medical Center, University of California, San Francisco CA; bDepartment of Pathology, Children’s Memorial Hospital, Chicago IL; cDepartment of Pathology, University of Virginia Health Science Center, Charlottesville VA (USA)
The gonadoblastoma locus on the Y chromosome (GBY) predisposes the dysgenetic gonads of XY females to develop in situ tumors. It has been mapped to a critical interval on the short arm and adjacent centromeric region on the Y chromosome. Currently there are five functional genes identified on the GBY critical region, thereby providing likely candidates for this cancer predisposition locus. To evaluate the candidacy of one of these five genes, testis-specific protein Y-encoded (TSPY), as the gene for GBY, expression patterns of TSPY in four gonadoblastoma from three patients were analyzed by immunohistochemistry using a TSPY specific antibody. Results from this study showed that TSPY was preferentially expressed in tumor germ cells of all gonadoblastoma specimens. Additional study on two cases of testicular seminoma demonstrated that TSPY was also abundantly expressed in all stages of these germ cell tumors. The present observations suggest that TSPY may either be involved in the oncogenesis of or be a useful marker for both types of germ cell tumors.
Request reprints from Dr. Chris Lau, Division of Cell and Developmental Genetics, Department of Medicine, VA Medical Center, 111C5, 4150 Clement Street, San Francisco, CA 94121 (USA); telephone: (415) 476-8839; fax: (415) 750-6633; e-mail: firstname.lastname@example.org
This work was partially supported by research grants from the National Institutes of Health, Department of Veterans Affairs and the Department of Defense Prostate Cancer Research Program (to Y.-F.C.L.). Y.-F.C.L. is a Senior Research Career Scientist of the Department of Veterans Affairs, USA.
Received: Received 7 August 2000;
manuscript accepted 17 August 2000.
Number of Print Pages : 5
Number of Figures : 1, Number of Tables : 0, Number of References : 35
Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger
Vol. 91, No. 1-4, Year 2000 (Cover Date: 2000)
Journal Editor: H.P. Klinger, Bronx, N.Y.; M. Schmid, Würzburg
ISSN: 0301–0171 (print), 1422–9816 (Online)
For additional information: http://www.karger.com/journals/ccg