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Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9Svensson A-C.a · Raudsepp T.b · Larsson C.c · Di Cristofano A.d · Chowdhary B.e · La Mantia G.f · Rask L.g · Andersson G.h
aUppsala Genetic Center, Swedish University of Agricultural Sciences, Uppsala (Sweden); bDivision of Animal Genetics, Royal Veterinary and Agricultural University, Frederiksberg (Denmark); cDepartment of Molecular Medicine, Karolinska Hospital, Stockholm (Sweden); dDepartment of Human Genetics, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York NY; eDepartment of Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, TX (USA); fDepartment of Genetics, General and Molecular Biology, University of Naples, Naples (Italy); gDepartment of Medical Biochemistry and Microbiology, Uppsala University, Uppsala; hDepartment of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Uppsala (Sweden)
ERV9 is a class I family of human endogenous retroviral sequences. Somatic cell hybrid genomic hybridization experiments using a mono-chromosomal panel indicate the presence of approximately 120 ERV9 loci in the human genome distributed on most chromosomes. Fluorescence in situ hybridization (FISH) using an ERV9 cDNA probe containing gag, pol and env sequences, verified this observation and a consistent signal was found at the chromosome region 11q13.3→q13.5. By analysis of a panel of radiation hybrids, an ERV9 locus was mapped to within a 300-kbp region at the chromosome site 11q13. The marker cCLGW567 and the locus MAP3K11/D11S546 centromeric and telomeric flanked it, respectively. Northern blot analysis, using an ERV9 LTR probe, indicated that most normal tissues examined expressed low abundant ERV9 LTR driven mRNAs of various sizes. The most prominent expression was found in adrenal glands and testis. However, the level of expression varied in the same tissues among different individuals indicating that ERV9 mRNA expression probably is inducible in certain tissues or at various cell stages.
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