Reconstruction of the female Gorilla gorilla karyotype using 25-color FISH and multicolor banding (MCB)Mrasek K.a · Heller A.a · Rubtsov N.b · Trifonov V.b · Starke H.a · Rocchi M.c · Claussen U.a · Liehr T.a
aInstitute of Human Genetics and Anthropology, Jena (Germany); bInstitute of Cytology and Genetics, Novosibirsk (Russia); and cInstitute of Genetics, Bari (Italy)
The origin of the human and great ape chromosomes has been studied by comparative chromosome banding analysis and, more recently, by fluorescence in situ hybridization (FISH), using human whole-chromosome painting probes. It is not always possible, however, to determine the exact breakpoints and distribution or orientation of specific DNA regions using these techniques. To overcome this problem, the recently developed multicolor banding (MCB) probe set for all human chromosomes was applied in the present study to reanalyze the chromosomes of Gorilla gorilla (GGO). While the results agree with those of most previous banding and FISH studies, the breakpoints for the pericentric inversion on GGO 3 were defined more precisely. Moreover, no paracentric inversion was found on GGO 14, and no pericentric inversions could be demonstrated on GGO 16 or 17.
© 2001 S. Karger AG, Basel
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Supported, in part, by the Herbert Quandt Stiftung der VARTA AG, the Madeleine Schickedanz-Kinderkrebs-Stiftung, the Deutscher Akademischer Austauschdienst (DAAD), the Deutsche Forschungsgemeinschaft (Grant No. 436 RUS 17/40/00), and the Wilhelm Sander-Stiftung (Grant No. 99.105.1). The authors gratefully acknowledge the financial support of Carl Zeiss Jena GmbH for color printing.
Received: Received 4 April 2001;
accepted 8 May 2001.
Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 0, Number of References : 30
Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger
Vol. 93, No. 3-4, Year 2001 (Cover Date: 2001)
Journal Editor: H.P. Klinger, Bronx, N.Y.; M. Schmid, Würzburg
ISSN: 0301–0171 (print), 1422–9816 (Online)
For additional information: http://www.karger.com/journals/ccg