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Table of Contents
Vol. 128, No. 2, 2002
Issue release date: June 2002
Section title: Original Paper
Int Arch Allergy Immunol 2002;128:105–114
(DOI:10.1159/000059400)

IgE Reactivity to Profilin in Pollen-Sensitized Subjects with Adverse Reactions to Banana and Pineapple

Reindl J.a · Rihs H.P.b · Scheurer S.a · Wangorsch A.a · Haustein D.a · Vieths S.a
aDepartment of Allergology, Paul-Ehrlich-Institut, Langen, and bResearch Institute for Occupational Medicine at the Ruhr University, Bochum, Germany

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 13, 2002
Issue release date: June 2002

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: The so-called ‘latex-fruit syndrome’ is a well-documented phenomenon in cross-reactive allergies. By contrast, there is a lack of information about allergy to exotic fruits in patients with a predominant pollen sensitization. Since the ubiquitous protein profilin has been identified as an allergen in natural rubber latex as well as in pollen-related foods, the aim of this study was to investigate the role of profilin in allergy to certain exotic fruits. Methods: Recombinant profilins from banana and pineapple were cloned by a PCR technique after isolation of total RNA using degenerated profilin-specific primers. The unknown 5′ ends of copy DNA (cDNA) were identified by rapid amplification of 5′cDNA ends (5′-RACE) and expression in Escherichia coli BL21(DE3) cells. The recombinant profilins were purified by affinity chromatography using poly-(L)-proline as the solid phase. IgE-binding capabilities were characterized by means of immunoblot and Enzyme Allergosorbent Test (EAST). The cross-reactivity to birch pollen profilin and latex profilin was studied by EAST as well as by immunoblot inhibition experiments. Results: Both banana and pineapple profilin were found to consist of 131 amino acid residues with high amino acid sequence identity to known allergenic pollen and food profilins (71–84%). IgE binding to the recombinant profilins was observed in 7/16 sera from subjects with suspected banana allergy (44%) and in 8/19 sera from subjects with suspected pineapple allergy (42%). Inhibition experiments indicated similar IgE reactivity of natural and recombinant allergens. In addition, high cross-reactivity to birch pollen profilin Bet v 2 and latex profilin Hev b 8 was demonstrated by immunoblot inhibition as well as EAST inhibition experiments. Conclusions: Since a high IgE-binding prevalence of about 40% was obtained in both banana and pineapple allergy, we conclude that profilin is an important mediator of IgE cross-reactivity between pollen and exotic fruits.

© 2002 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: June 13, 2002
Issue release date: June 2002

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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