Journal Mobile Options
Table of Contents
Vol. 23, No. 2, 2002
Issue release date: March–April 2002 (June 2002)
Section title: Review
Tumor Biol 2002;23:103–112
(DOI:10.1159/000059713)

Photochemical Internalisation: A Novel Drug Delivery System

Selbo P.K.a · Høgset A.b · Prasmickaite L.a · Berg K.a
aDepartment of Biophysics, Institute for Cancer Research, Norwegian Radium Hospital, bPCI Biotech AS, Oslo,Norway

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

For eJournal Archive and eJournal Backfiles information please contact service@karger.com


Article / Publication Details

First-Page Preview
Abstract of Review

Published online: 6/17/2002
Issue release date: March–April 2002 (June 2002)

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI

Abstract

The present report reviews a number of recently published papers on a novel technology for the cytosolic delivery of macromolecules named photochemical internalisation (PCI). PCI is based upon the light activation of a drug (a photosensitiser) specifically located in the membrane of endocytic vesicles. Light which is absorbed by the photosensitiser induces the formation of reactive oxygen species, of which singlet oxygen (1O2) is the predominant form. Singlet oxygen oxidises biomolecules in the membranes of endosomes and lysosomes, resulting in a subsequent release of the contents of these compartments into the cytosol. Photosensitisers have a higher affinity for tumour tissues than for most normal tissues and are used in photodynamic therapy of various types of cancers. We have taken advantage of the PCI strategy to enhance the delivery of a variety of macromolecules, including ribosome-inactivating toxins, an immunotoxin, horse radish peroxidase, a ras peptide, RNA, oligonucleotides and protein encoding DNA, to the cytosol. Normally, a major intracellular barrier to the application of therapeutically interesting peptides and proteins or the application of DNA and RNA in gene therapy is the degradation of the macromolecules in the endocytic vesicles after uptake by endocytosis. Therefore, a photochemically induced rupture of endocytic vesicles and the subsequent cytosolic release of the macromolecules aids these molecules in escaping attack by the lysosomal hydrolases, thereby maintaining their biological activity. Thus, PCI represents a novel principle for the cytosolic delivery of biologically active macromolecules which overcomes the pivotal intracellular barrier of endosomes and lysosomes. In addition to being utilised as a new site-specific cancer therapy method, PCI can also be applied as a research tool for macromolecule delivery both in vitro and in vivo.

© 2002 S. Karger AG, Basel


  

Author Contacts

Dr. Pål Kristian Selbo
Department of Biophysics, Institute for Cancer Research
Norwegian Radium Hospital, Montebello
N–0310 Oslo 3 (Norway)
Tel. +47 22 93 42 61, Fax +47 22 93 42 70, E-Mail p.k.selbo@labmed.uio.no

  

Article Information

Received: Received: December 29, 2001
Accepted after revision: March 12, 2002
Number of Print Pages : 10
Number of Figures : 5, Number of Tables : 2, Number of References : 64

  

Publication Details

Tumor Biology (Tumor Markers and Translational Cancer Research)
Founded 1980 as ‘Oncodevelopmental Biology and Medicine’ by the ISOBM, continued 1984–1986 as ‘Tumour Biology’
The Journal of the International Society for Oncodevelopmental Biology and Medicine (ISOBM)

Vol. 23, No. 2, Year 2002 (Cover Date: March-April 2002 (Released June 2002))

Journal Editor: T. Stigbrand, Umeå; Managing Editor: P.D. Rye, Oslo
ISSN: 1010–4283 (print), 1423–0380 (Online)

For additional information: http://www.karger.com/journals/tbi


Article / Publication Details

First-Page Preview
Abstract of Review

Published online: 6/17/2002
Issue release date: March–April 2002 (June 2002)

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.