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Vol. 9, No. 4, 2002
Issue release date: July–August 2002
Section title: Original Paper
J Biomed Sci 2002;9:339–347
(DOI:10.1159/000065005)

Characterization of Contractile Function and Expression of Muscarinic Receptors, G Proteins and Adenylate Cyclase in Cultured Tracheal Smooth Muscle of Swine

Hsieh J.-T. · Farley J.M.
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Miss., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/19/2002
Issue release date: July–August 2002

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS

Abstract

Smooth muscle cells lose their contractile function and phenotype very rapidly when placed in culture. During organ culture of smooth muscle strips, phenotype is lost more slowly. In the present studies, we established an organ culture model to study contractile function and expression of muscarinic receptors, G proteins and adenylyl cyclase in different serum concentrations in tracheal smooth muscle from swine. The results show that contractile function and the amounts of M3 receptors, G proteins and adenylyl cyclase were maintained for up to 5 days in culture. The expression of M2 receptors was significantly decreased in culture when compared to freshly isolated muscles. Maximal isometric tension was significantly increased in cultured muscles compared with freshly isolated muscles. Different serum concentrations did not significantly affect contractile function and expression of muscarinic receptors, G proteins and adenylyl cyclase. In conclusion, our studies suggest that cultured smooth muscle might be used as a model to study the regulation of contractile function of smooth muscle by various signal transduction pathways.

© 2002 National Science Council, ROC and S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/19/2002
Issue release date: July–August 2002

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS


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