Characterization of Contractile Function and Expression of Muscarinic Receptors, G Proteins and Adenylate Cyclase in Cultured Tracheal Smooth Muscle of SwineHsieh J.-T. · Farley J.M.
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Miss., USA
Smooth muscle cells lose their contractile function and phenotype very rapidly when placed in culture. During organ culture of smooth muscle strips, phenotype is lost more slowly. In the present studies, we established an organ culture model to study contractile function and expression of muscarinic receptors, G proteins and adenylyl cyclase in different serum concentrations in tracheal smooth muscle from swine. The results show that contractile function and the amounts of M3 receptors, G proteins and adenylyl cyclase were maintained for up to 5 days in culture. The expression of M2 receptors was significantly decreased in culture when compared to freshly isolated muscles. Maximal isometric tension was significantly increased in cultured muscles compared with freshly isolated muscles. Different serum concentrations did not significantly affect contractile function and expression of muscarinic receptors, G proteins and adenylyl cyclase. In conclusion, our studies suggest that cultured smooth muscle might be used as a model to study the regulation of contractile function of smooth muscle by various signal transduction pathways.
© 2002 National Science Council, ROC and S. Karger AG, Basel
Received: Received: October 19, 2001
Accepted: February 4, 2002
Number of Print Pages : 9
Number of Figures : 3, Number of Tables : 2, Number of References : 60
Journal of Biomedical Science (Sponsored by the National Science Council, Taipei)
Vol. 9, No. 4, Year 2002 (Cover Date: July-August 2002)
Journal Editor: S.H.H.Chan, Kaohsiung
ISSN: 1021–7770 (print), 1423–0127 (Online)
For additional information: http://www.karger.com/journals/jbs