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Vol. 129, No. 1, 2002
Issue release date: September 2002
Section title: Short Communication
Int Arch Allergy Immunol 2002;129:93–96
(DOI:10.1159/000065178)

Identification of Sequential IgE-Binding Epitopes on Bovine αs2-Casein in Cow’s Milk Allergic Patients

Busse P.J. · Järvinen K.M. · Vila L. · Beyer K. · Sampson H.A.
Department of Pediatrics, Division of Allergy and Immunology, and Jaffe Institute for Food Allergy, The Mount Sinai School of Medicine, New York, N.Y., USA

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Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 11/27/2001
Accepted: 4/7/2002
Published online: 9/30/2002

Number of Print Pages: 4
Number of Figures: 2
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: Caseins are the major allergens responsible for cow’s milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow’s milk (CM) proteins except for αs2-casein. Methods: Overlapping decapeptides representing the entire length of αs2-casein were synthesized on a cellulose-derivatized membrane. Sera from 13 CM-allergic children, 4–15 years of age, with a median level of CM-specific IgE >100 kU/l (range 33.7 to > 100 kU/l) were used to identify IgE-binding epitopes. Results: Four major and six minor sequential IgE-binding regions were identified on αs2-casein. The first major region is located in the middle of the protein at amino acids (AA) 83–100, and the other three major regions are located in the carboxy terminal portion of the protein at AA 143–158, 157–172 and 165–188. The minor IgE-binding regions were identified at AA 31–44, 43–56, 93–106, 105–114, 117–128, and 191–200. Conclusion: We identified 10 sequential IgE-binding regions on αs2-casein and performed the first crucial step in the development of immunotherapeutic interventions for CMA.


  

Author Contacts

Correspondence to: Dr. Hugh A. Sampson
Department of Pediatrics, Box 1198, The Mount Sinai School of Medicine
1 Gustave L. Levy Place
New York, NY 10029-6574 (USA)
Tel. +1 212 241 5243, Fax +1 212 426 1902, E-Mail Hugh.Sampson@mssm.edu

  

Article Information

Received: Received: November 27, 2001
Accepted after revision: April 7, 2002
Number of Print Pages : 4
Number of Figures : 2, Number of Tables : 0, Number of References : 9

  

Publication Details

International Archives of Allergy and Immunology
Founded 1950

Vol. 129, No. 1, Year 2002 (Cover Date: September 2002)

Journal Editor: D. Kraft, Vienna
ISSN: 1018–2438 (print), 1423–0097 (Online)

For additional information:http://www.karger.com/journals/iaa


Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 11/27/2001
Accepted: 4/7/2002
Published online: 9/30/2002

Number of Print Pages: 4
Number of Figures: 2
Number of Tables: 0

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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