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Vol. 22, No. 5-6, 2002
Issue release date: September–December 2002
Section title: Laboratory Investigation
Am J Nephrol 2002;22:587–595
(DOI:10.1159/000065284)

Apoptotic Cell Loss following Cell Proliferation in Renal Glomeruli of Otsuka Long-Evans Tokushima Fatty Rats, a Model of Human Type 2 Diabetes

Murata I.a · Takemura G.a · Asano K.a · Sano H.a · Fujisawa K.a · Kagawa T.a · Baba K.a · Maruyama R.a · Minatoguchi S.a · Fujiwara T.b · Fujiwara H.a
aSecond Department of Internal Medicine, Gifu University School of Medicine, Gifu, and bDepartment of Food Science, Kyoto Women’s University, Kyoto, Japan

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Article / Publication Details

First-Page Preview
Abstract of Laboratory Investigation

Received: 1/7/2002
Accepted: 6/10/2002
Published online: 10/7/2002
Issue release date: September–December 2002

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN

Abstract

Background: The mechanism of glomerular cell loss during the late stage of diabetic nephropathy is unknown. Methods: We examined cell population, proliferation, apoptosis, and immunohistochemical expression of apoptosis-related proteins, Bcl-2 and Bax, in renal glomeruli of the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, an animal model of human type 2 diabetes. 10-, 30-, 50-, and 70-week-old rats were used (n = 5–8). Control was the Long-Evans Tokushima Otsuka (LETO) rat. Results: The cell population in renal glomeruli of OLETF rats progressively increased with age, but decreased at 70 weeks old. High cell proliferative activity based on proliferating cell nuclear antigen (PCNA) expression was limited during the early stage, whereas by in situ nick end-labeling (TUNEL), Taq polymerase based in situ ligation, and electron microscopy, apoptosis was detected during the late stage (50 and 70 weeks old). Augmented expression of Bax, but not of Bcl-2, was evident in glomeruli of OLETF rats during the late stage, which contributed to an increased Bax/Bcl-2 ratio. Conclusion: It appears that high cell proliferative activity and the subsequent cell loss via apoptosis counterbalance each other and determine glomerular cell population of OLETF rats. Augmented Bax expression may be one of the important regulators of this apoptosis.

© 2002 S. Karger AG, Basel


  

Author Contacts

Hisayoshi Fujiwara, MD, PhD
Second Department of Internal Medicine
Gifu University School of Medicine
40 Tsukasa-Machi, Gifu 500-8705 (Japan)
Tel. +81 58 267 2257, Fax +81 58 267 2933, E-Mail gifuim-gif@umin.ac.jp

  

Article Information

Received: Received: January 7, 2002
Accepted: June 10, 2002
Number of Print Pages : 9
Number of Figures : 6, Number of Tables : 1, Number of References : 26

  

Publication Details

American Journal of Nephrology

Vol. 22, No. 5-6, Year 2002 (Cover Date: September-December 2002)

Journal Editor: Shaul G. Massry, Los Angeles, California
ISSN: 0250–8095 (print), 1421–9670 (Online)

For additional information: http://www.karger.com/journals/ajn


Article / Publication Details

First-Page Preview
Abstract of Laboratory Investigation

Received: 1/7/2002
Accepted: 6/10/2002
Published online: 10/7/2002
Issue release date: September–December 2002

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN


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