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Vol. 39, No. 4, 2002
Issue release date: July–August 2002
Section title: Internet Discussion Forum
J Vasc Res 2002;39:304–310
(DOI:10.1159/000065542)

Differential Expression of Genes from Nitrate-Tolerant Rat Aorta

Pi X. · Yan C. · Kim D. · Chen J. · Berk B.C.
Center for Cardiovascular Research, Medical Center, University of Rochester, N.Y., USA

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Article / Publication Details

First-Page Preview
Abstract of Internet Discussion Forum

Published online: 8/12/2002

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance including alterations in gene expression. To identify genes whose expression is altered due to chronic treatment with nitroglycerin (NTG), nitrate-tolerant rats were made by infusion of NTG for 3 days (10 µg/kg/min) using an osmotic minipump. We constructed a PCR-selected cDNA subtracted library from NTG-treated and vehicle-treated rat aortas. Screening of ∼500 clones in the subtracted library showed that four genes were regulated by NTG treatment. Specifically, mRNA levels of β-globin, tropoelastin, gelsolin and a small G protein were confirmed to be upregulated consistently by NTG treatment. These identified genes may play important roles in the development of nitrate tolerance and represent promising candidates to understand the mechanisms of nitrate tolerance and endothelial dysfunction in the vasculature.


  

Author Contacts

Dr. Bradford C. Berk
Department of Medicine
Box MED, University of Rochester School of Medicine
601 Elmwood Avenue, Rochester, NY 14642 (USA)
Tel. +1 585 275 0810, Fax +1 585 273 1497, E-Mail Bradford_Berk@urmc.rochester.edu

  

Article Information

Received: Received: October 11, 2001
Accepted after revision: January 7, 2002
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 2, Number of References : 29

  

Publication Details

Journal of Vascular Research
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)

Vol. 39, No. 4, Year 2002 (Cover Date: July-August 2002)

Journal Editor: M.J. Mulvany, Aarhus
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/journals/jvr


Article / Publication Details

First-Page Preview
Abstract of Internet Discussion Forum

Published online: 8/12/2002

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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