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Vol. 45, No. 3, 2002
Issue release date: May–June 2002 (October 2002)
Section title: Original Paper
Intervirology 2002;45:136–141
(DOI:10.1159/000065866)

Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis

Ishiko H.a · Miura R.a · Shimada Y.a · Hayashi A.a · Nakajima H.a · Yamazaki S.a · Takeda N.b
aResearch and Development Department, Mitsubishi Kagaku Bio-Clinical Laboratories, and bDepartment of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/22/2002
Accepted: 5/1/2002
Published online: 10/30/2002
Issue release date: May–June 2002 (October 2002)

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 0

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: http://www.karger.com/INT

Abstract

Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-bp fragment. The single exception was HRV-87, which generated a 650-bp fragment, as observed in human EVs. The VP4 nucleotide sequence of HRV-87 showed more than 97% nucleotide identity with human EV-68, and formed a monophyletic cluster along with the prototype strain of EV-68 in the human EV-D cluster. HRV-87 showed the second highest homology (76.8%) with EV-70, another member of the human EV-D, in a sample of 66 human EVs and 12 HRVs. Therefore, HRV-87 should be reclassified into the cluster containing human EV-68.

© 2002 S. Karger AG, Basel


  

Author Contacts

Hiroaki Ishiko, PhD
Research and Development Department
Mitsubishi Kagaku Bio-Clinical Laboratories Inc.
Shimura 3-30-1, Itabashi, Tokyo 174-8555 (Japan)
Tel. +81 3 5994 2332, Fax +81 3 5994 2972, E-Mail Ishiko@m1.mbcl.co.jp

  

Article Information

Received: Received: April 22, 2002
Accepted after revision: May 1, 2002
Number of Figures : 3, Number of Tables : 0, Number of References : 14

  

Publication Details

Intervirology (International Journal of Basic and Medical Virology)
Founded 1973 by J.L. Melnick; continued by F. Rapp (1986–1990); M.J. Buchmeier and C.R. Howard (1991–1993)

Vol. 45, No. 3, Year 2002 (Cover Date: May-June 2002 (Released October 2002))

Journal Editor: Rüdiger W. Braun, Stuttgart
ISSN: 0300–5526 (print), 1423–0100 (Online)

For additional information: http://www.karger.com/journals/int


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/22/2002
Accepted: 5/1/2002
Published online: 10/30/2002
Issue release date: May–June 2002 (October 2002)

Number of Print Pages: 6
Number of Figures: 3
Number of Tables: 0

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: http://www.karger.com/INT


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