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Table of Contents
Vol. 172, No. 3, 2002
Issue release date: 2002
Section title: Paper
Cells Tissues Organs 2002;172:174–189
(DOI:10.1159/000066964)

Measuring Cell Adhesion Forces with the Atomic Force Microscope at the Molecular Level

Benoit M. · Gaub H.E.
Center for Nano Science, Ludwigs-Maximilians-Universität, Munich, Germany

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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 10, 2002
Published online: December 18, 2002
Issue release date: 2002

Number of Print Pages: 16
Number of Figures: 12
Number of Tables: 2

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

In the past 25 years many techniques have been developed to characterize cell adhesion and to quantify adhesion forces. Atomic force microscopy (AFM) has been used to measure forces in the pico-newton range, an experimental technique known as force spectroscopy. We modified such an AFM to measure adhesion forces between live cells or between cells and surfaces. This strategy required functionalizing the surface of the sensors for immobilizing the cell. We used Dictyostelium discoideum cells which respond to starvation by surface expression of the adhesion molecule csA and consequent aggregation to measure the adhesion force of a single csA-csA bond. Relevant experimental parameters include the duration of contact between the interacting surfaces, the force against which this contact is maintained, the number and specificity of interacting adhesion molecules and the constituents of the medium in which the interaction occurs. This technology also permits the measurement of the viscoelastic properties of single cells or cell layers.

© 2002 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Paper

Received: September 10, 2002
Published online: December 18, 2002
Issue release date: 2002

Number of Print Pages: 16
Number of Figures: 12
Number of Tables: 2

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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